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Table of Contents
Part I: Super-Resolution Microscopy and Molecular Imaging Techniques to Probe Biology
1. Introduction on Single-Molecule Science
2. One Molecule, Two Molecules, Red Molecules, Blue Molecules: Methods for Quantifying Localization Microscopy Data
3. Multiscale Fluorescence Imaging
4. Long-Read Single-Molecule Optical Maps
Part II: Protein Folding, Structure, Confirmation, and Dynamics
5. Single-Molecule Mechanics of Protein Nanomachines
6. Posttranslational Protein Translocation through Membranes at the Single-Molecule Level
Part III: Mapping DNA Molecules at the Single-Molecule Level
7. Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope
8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification
Part IV: Single-Molecule Biology to Study Gene Expression
9. Single-Molecule Detection in the Study of Gene Expression
1. Introduction on Single-Molecule Science
2. One Molecule, Two Molecules, Red Molecules, Blue Molecules: Methods for Quantifying Localization Microscopy Data
3. Multiscale Fluorescence Imaging
4. Long-Read Single-Molecule Optical Maps
Part II: Protein Folding, Structure, Confirmation, and Dynamics
5. Single-Molecule Mechanics of Protein Nanomachines
6. Posttranslational Protein Translocation through Membranes at the Single-Molecule Level
Part III: Mapping DNA Molecules at the Single-Molecule Level
7. Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope
8. Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification
Part IV: Single-Molecule Biology to Study Gene Expression
9. Single-Molecule Detection in the Study of Gene Expression