2.8 MRV 1-like Cell Attachment Protein of GCRV-II Species
2.9 Conclusions and Future Considerations
References
Chapter 3: The Aquareovirus Particle Structure and Protein Functions
3.1 Introduction
3.2 The Aquareovirus Particle and Its Structural Protein Localization
3.2.1 Similarities and Differences between the Aquareovirus, Orthoreovirus, and Other Viruses in the Family Reoviridae
3.2.2 Comparison of Structural Proteins in the Aquareoviruses and Orthoreoviruses
3.3 Cryo-EM Analysis of the Aquareovirus/GCRV Virions

Intro
Preface
Contents
Contributors
Chapter 1: Aquareovirus: An Overview
1.1 Introduction
1.2 Origin of AqRV
1.3 Characteristic Features of AqRV
1.4 Taxonomy and Classification of the Genus AqRV
1.5 Geographic and Seasonal Distribution
1.6 Host Range and Viral Proliferation
1.7 Antigenic and Serological Characterization of AqRV
1.8 Representative and Pathogenic AqRV Strains
1.9 Conclusions and Future Considerations
References
Chapter 2: Molecular Biology of Aquareoviruses
2.1 Introduction
2.2 Particle-Based Properties

2.2.1 Virus Proliferation and Sample Preparation
2.2.2 Different Particle Components Extracted from Purified Virus Preparations
2.2.2.1 Intact Virions and Empty Particles
2.2.2.2 SVP and Virus Core
2.2.3 The Virus-Antibody Immunocompound of GCRV
2.2.4 Recombinant GCRV Particles
2.2.5 Surface Labeling of AqRV Particles
2.2.6 Infectivity Assays of Purified or Modified Virions and SVPs
2.3 AqRV Structural Protein Profile
2.3.1 Identification of AqRV Particle Proteins
2.3.2 Various Particle Forms and Related Protein Profiles

2.4 In Vitro Endogenous Transcription Assays of GCRV Particles
2.4.1 GCRV Nucleic Acid Release
2.4.2 GCRV Reaction Core
2.4.3 In Vitro Transcription Assays
2.5 The AqRV Genome
2.5.1 Nomenclature and Electrophoresis Profile of the AqRV dsRNA Genome
2.5.2 Genome Sequence-Based Features of AqRVs
2.5.3 Terminal Non-coding Regions
2.5.4 Protein-Coding Assignments and Nomenclature
2.5.4.1 SBRV Gene-Protein-Coding Assignment
2.5.4.2 GCRV Gene-Protein-Coding Assignment
2.5.5 In Vitro and In Vivo Protein Expression Analyses
2.6 AqRV Morphogenesis

2.6.1 TEM Analysis of VIB Formation in Infected Cells
2.6.2 NS80: A Scaffold of VIBs
2.6.3 Interaction of NS80 with Viral Proteins
2.6.4 Role of NS38 in GCRV Morphogenesis
2.7 GCRV S7 Genome Segment-Encoded FAST Protein and Associated Syncytium Formation
2.7.1 Detection of FAST Protein
2.7.2 Sequence-Based Features of FAST Protein
2.7.3 NS16 and NS22: The AqRV FAST Proteins
2.7.4 NS12: A Novel Membrane-Associated Protein
2.7.5 Role of NS31 in Virus Replication
2.7.6 Possible Function of GCRV NS26 in Syncytia Formation