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001438679 001__ 1438679
001438679 003__ OCoLC
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001438679 019__ $$a1263024919
001438679 020__ $$a9783030646868$$q(electronic bk.)
001438679 020__ $$a3030646866$$q(electronic bk.)
001438679 020__ $$z3030646858
001438679 020__ $$z9783030646851
001438679 0247_ $$a10.1007/978-3-030-64686-8$$2doi
001438679 035__ $$aSP(OCoLC)1262726532
001438679 040__ $$aYDX$$beng$$epn$$cYDX$$dGW5XE$$dEBLCP$$dOCLCO$$dOCLCF$$dUKAHL$$dOCLCO$$dHF9$$dOCLCQ$$dCOM$$dOCLCO$$dOCLCQ$$dN$T$$dOCLCQ
001438679 049__ $$aISEA
001438679 050_4 $$aQR181
001438679 08204 $$a571.9/6$$223
001438679 1001_ $$aSam-Yellowe, Tobili Y.
001438679 24510 $$aImmunology :$$boverview and laboratory manual /$$cTobili Y. Sam-Yellowe.
001438679 260__ $$aCham, Switzerland :$$bSpringer,$$c2021.
001438679 300__ $$a1 online resource
001438679 336__ $$atext$$btxt$$2rdacontent
001438679 337__ $$acomputer$$bc$$2rdamedia
001438679 338__ $$aonline resource$$bcr$$2rdacarrier
001438679 504__ $$aIncludes bibliographical references and index.
001438679 5050_ $$aPart I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual -- 27. Immunology laboratory policies -- 28. Instructions for notebook entries -- 29. Instructions for writing lab reports -- 30. Lab report topics -- 31. Exercise 1: Using a compound microscope -- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- 43. EXERCISE 13: Immunoblotting (Western Blotting) -- 44. Exercise 14: Protein Assay -- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- 47. EXERCISE 17: Immunofluorescence assay (IFA) -- 48. EXERCISE 18: Flow Cytometry -- 49. EXERCISE 19: Complement Fixation Assays -- 50. Tissue Cell Culture Techniques -- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- 54. EXERCISE 23: Macrophage Migration Inhibition -- 55. EXERCISE 24: Monoclonal Antibody Preparation -- 56. EXERCISE 25: Immunization of mice with a recombinant protein -- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- 58. Exercise 27: Immunoprecipitation -- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies -- Appendix -- Sample Practice Questions.
001438679 506__ $$aAccess limited to authorized users.
001438679 520__ $$aA two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
001438679 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed August 16, 2021).
001438679 650_0 $$aImmunology.
001438679 650_0 $$aImmunology$$vLaboratory manuals.
001438679 650_6 $$aImmunologie.
001438679 650_6 $$aImmunologie$$vManuels de laboratoire.
001438679 655_7 $$aLaboratory manuals.$$2fast$$0(OCoLC)fst01920776
001438679 655_7 $$aLaboratory manuals.$$2lcgft
001438679 655_7 $$aManuels de laboratoire.$$2rvmgf
001438679 655_0 $$aElectronic books.
001438679 77608 $$iPrint version:$$aSam-Yellowe, Tobili Y.$$tImmunology.$$dCham, Switzerland : Springer, 2021$$z3030646858$$z9783030646851$$w(OCoLC)1202755493
001438679 852__ $$bebk
001438679 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-3-030-64686-8$$zOnline Access$$91397441.1
001438679 909CO $$ooai:library.usi.edu:1438679$$pGLOBAL_SET
001438679 980__ $$aBIB
001438679 980__ $$aEBOOK
001438679 982__ $$aEbook
001438679 983__ $$aOnline
001438679 994__ $$a92$$bISE