@article{1439735,
      author = {Zaheer, Sameer Mahmood, and Gosu, Ramachandraiah,},
      url = {http://library.usi.edu/record/1439735},
      title = {Methods for fragments screening using surface plasmon  resonance /},
      abstract = {This volume describes methods and protocols for the  fragment-based screening of proteins using Surface Plasmon  Resonance (SPR). The initial chapter of the book discusses  the principle of SPR for the identification of biomolecular  interactions, while the subsequent chapters introduce  methods for labelling proteins with different tags  including, histidine and biotin tags. It also discusses  techniques and factors that affect the amine and  biotin-streptavidin coupling and methods to optimize the  interactions. Next, it describes fragment preparation for  screening in SPR and presents methods to calculate  equilibrium dissociation constant (KD) and ligand  efficiency (LE). It reviews techniques of next-generation  injections that improve the efficiency of the  characterization process over traditional SPR by  determining the kinetics and affinity in a single step.  Finally, the book elucidates a comprehensive yet  representative description of challenges associated with  the molecular interaction of proteins using SPR.},
      doi = {https://doi.org/10.1007/978-981-16-1536-8},
      recid = {1439735},
      pages = {1 online resource :},
}