001439735 000__ 04038cam\a2200577\i\4500
001439735 001__ 1439735
001439735 003__ OCoLC
001439735 005__ 20230309004518.0
001439735 006__ m\\\\\o\\d\\\\\\\\
001439735 007__ cr\un\nnnunnun
001439735 008__ 210918s2021\\\\si\a\\\\o\\\\\000\0\eng\d
001439735 019__ $$a1268574321$$a1284941152
001439735 020__ $$a9789811615368$$q(electronic bk.)
001439735 020__ $$a9811615365$$q(electronic bk.)
001439735 020__ $$z9789811615351
001439735 020__ $$z9811615357
001439735 0247_ $$a10.1007/978-981-16-1536-8$$2doi
001439735 035__ $$aSP(OCoLC)1268360811
001439735 040__ $$aYDX$$beng$$erda$$epn$$cYDX$$dGW5XE$$dOCLCO$$dEBLCP$$dDKU$$dOCLCF$$dUKAHL$$dOCLCO$$dOCLCQ$$dCOM$$dOCLCQ
001439735 049__ $$aISEA
001439735 050_4 $$aQP551.5$$b.M48 2021
001439735 08204 $$a572/.636$$223
001439735 24500 $$aMethods for fragments screening using surface plasmon resonance /$$cSameer Mahmood Zaheer, Ramachandraiah Gosu, editors.
001439735 264_1 $$aSingapore :$$bSpringer,$$c[2021]
001439735 264_4 $$c©2021
001439735 300__ $$a1 online resource :$$billustrations (some color)
001439735 336__ $$atext$$btxt$$2rdacontent
001439735 337__ $$acomputer$$bc$$2rdamedia
001439735 338__ $$aonline resource$$bcr$$2rdacarrier
001439735 347__ $$atext file
001439735 347__ $$bPDF
001439735 5050_ $$aChapter 1. Introduction to Surface Plasmon Resonance -- Chapter 2. Principle of Surface Plasmon Resonance (OneStep) -- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling -- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture -- Chapter 5. Protein stability using Thermal Shift Assay (TSA) pH Tolerance -- Chapter 6. Protein stability using Thermal Shift Assay (TSA) DMSO Tolerance -- Chapter 7. Selection and Identification of Fragment library -- Chapter 8. Preparation of Sensor -- Chapter 9. Optimization and validation of Amine coupling of Ligand -- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand -- Chapter 11. Preparation of Fragments for Screening in SPR -- Chapter 12. Screening of Fragments in SPR -- Chapter 13. Identification of Fragment Hits -- Chapter 14. Data Analysis and Confirmation of Hits.
001439735 506__ $$aAccess limited to authorized users.
001439735 520__ $$aThis volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
001439735 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed September 23, 2021).
001439735 650_0 $$aProteins$$xAnalysis.
001439735 650_0 $$aProtein-protein interactions.
001439735 650_0 $$aSurface plasmon resonance.
001439735 650_6 $$aProtéines$$xAnalyse.
001439735 650_6 $$aInteractions protéine-protéine.
001439735 650_6 $$aRésonance plasmonique de surface.
001439735 655_0 $$aElectronic books.
001439735 7001_ $$aZaheer, Sameer Mahmood,$$eeditor.
001439735 7001_ $$aGosu, Ramachandraiah,$$eeditor.
001439735 77608 $$iPrint version:$$tMethods for fragments screening using surface plasmon resonance.$$dSingapore : Springer, [2021]$$z9811615357$$z9789811615351$$w(OCoLC)1240492502
001439735 852__ $$bebk
001439735 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-981-16-1536-8$$zOnline Access$$91397441.1
001439735 909CO $$ooai:library.usi.edu:1439735$$pGLOBAL_SET
001439735 980__ $$aBIB
001439735 980__ $$aEBOOK
001439735 982__ $$aEbook
001439735 983__ $$aOnline
001439735 994__ $$a92$$bISE