Methods for fragments screening using surface plasmon resonance / Sameer Mahmood Zaheer, Ramachandraiah Gosu, editors.
Zaheer, Sameer Mahmood, editor.; Gosu, Ramachandraiah, editor.
2021
QP551.5 .M48 2021
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Title
Methods for fragments screening using surface plasmon resonance / Sameer Mahmood Zaheer, Ramachandraiah Gosu, editors.
ISBN
9789811615368 (electronic bk.)
9811615365 (electronic bk.)
9789811615351
9811615357
DOI
https://doi.org/10.1007/978-981-16-1536-8
Published
Singapore : Springer, [2021]
Copyright
©2021
Language
English
Description
1 online resource : illustrations (some color)
Item Number
10.1007/978-981-16-1536-8 doi
Call Number
QP551.5 .M48 2021
Dewey Decimal Classification
572/.636
Summary
This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
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Source of Description
Online resource; title from PDF title page (SpringerLink, viewed September 23, 2021).
Added Author
Zaheer, Sameer Mahmood, editor.
Gosu, Ramachandraiah, editor.
Available in Other Form
Methods for fragments screening using surface plasmon resonance.
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Chapter 1. Introduction to Surface Plasmon Resonance
Chapter 2. Principle of Surface Plasmon Resonance (OneStep)
Chapter 3. Preparation of protein with Histidine Tag for Amine coupling
Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture
Chapter 5. Protein stability using Thermal Shift Assay (TSA) pH Tolerance
Chapter 6. Protein stability using Thermal Shift Assay (TSA) DMSO Tolerance
Chapter 7. Selection and Identification of Fragment library
Chapter 8. Preparation of Sensor
Chapter 9. Optimization and validation of Amine coupling of Ligand
Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand
Chapter 11. Preparation of Fragments for Screening in SPR
Chapter 12. Screening of Fragments in SPR
Chapter 13. Identification of Fragment Hits
Chapter 14. Data Analysis and Confirmation of Hits.

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