@article{1443611,
      recid = {1443611},
      author = {Hidaka, Takuya,},
      title = {Sequence-specific DNA binders for the therapy of  mitochondrial diseases /},
      pages = {1 online resource (104 pages) :},
      note = {"Doctoral thesis accepted by Kyoto University, Kyoto,  Japan."},
      abstract = {This book describes the authors work on the development of  sequence-specific DNA binders for the therapy of  mitochondrial diseases. In the first chapter, the author  provides a detailed background of pyrroleimidazole  polyamides (PIPs) and mitochondrial disease research  followed by chapters presenting the author's own research  and discoveries. Firstly, the developed compounds called  MITO-PIPs, which recognize specific sequences in  mitochondrial DNA, are presented. The following chapter  demonstrates how, by introducing a DNA alkylating reagent  into a MITO-PIP that recognizes the adjacent sequence to a  target mutation, the copy number of mutated mitochondrial  DNA was successfully reduced in live cells. Furthermore,  because nuclear DNA is another important target for  treating mitochondrial diseases, the author demonstrated  that tri-arginine vectors can enhance nuclear uptake of  PIPs and improve their biological activity in cells. This  work will attract readers interest because it paves the way  for a transgene-free chemical gene therapy for  mitochondrial diseases. The book includes a detailed  description of experimental procedures, especially compound  synthesis. This description helps readers to have a clear  image of the authors studies and to perform similar and  extended studies themselves.},
      url = {http://library.usi.edu/record/1443611},
      doi = {https://doi.org/10.1007/978-981-16-8436-4},
}