Intro
Preface
Reference
Contents
Chapter 1: First Generation Bioethanol: Fundamentals-Definition, History, Global Production, Evolution
1.1 Definition
1.2 History
1.3 Global Production
1.4 Contributions to Climate Change Mitigation and the Environment
1.5 Conclusion and Perspectives
References
Chapter 2: Feedstocks for First-Generation Bioethanol Production
2.1 Introduction
2.2 Production Feedstocks for 1G Ethanol Around Policy and Programs
2.3 Sugar-Based Feedstocks
2.3.1 Sugarcane
2.3.2 Sugar Beet
2.4 Starch-Based Feedstocks
2.4.1 Corn

2.4.2 Wheat
2.4.3 Cassava
2.5 Conclusion and Future Prospective
References
Chapter 3: Microorganisms and Genetic Improvement for First and Second Generation Bioethanol Production
3.1 Introduction
3.2 Concept of Fermentation Process for 1G and 2G Ethanol Production
3.3 Prospection and Isolation Strategies of Microorganisms for Ethanol Production
3.4 Diversity of Ethanol-Producing Microorganisms
3.5 Process-Driven Selection Strategy for Recovery of Ethanol-Producing Microorganisms
3.6 Classical Genetic Tools
3.6.1 Adaptive Evolution
3.6.2 Protoplast Fusion

3.6.3 Direct Mating
3.6.4 Mass Mating
3.6.5 Classical Genetic Tools
3.7 Pooled-Segregant Whole Genome Sequence Analysis
3.8 Deletion or Regulation of Genes
3.9 Whole-Genome Sequencing
3.10 CRISPR/Cas9
3.11 Concluding Remarks
References
Chapter 4: Enzymatic Hydrolysis of Feedstocks for 1G Bioethanol Production
4.1 Introduction
4.2 Starch Feedstock for 1G Bioethanol
4.2.1 Maize/Corn
4.2.2 Wheat
4.2.3 Cassava
4.2.4 Rice
4.2.5 Sorghum
4.2.6 Sweet Potato
4.3 Role of Enzymes in Starch Saccharification
4.4 Amylolytic Enzymes

4.4.1 Currently Used Amylases
4.5 Advanced Amylases
4.5.1 Engineered Strains for Consolidated Bioprocess
4.5.2 Alpha-Amylase Immobilization
4.6 Future Perspectives
4.6.1 Technological Advances
4.7 Conclusions
References
Chapter 5: Sugarcane First-Generation Bioethanol Units and Advancements in Electric Power and Biogas Production
5.1 Introduction
5.2 1G Bioethanol Production Out of Sugarcane
5.2.1 Sugarcane Planting and Harvesting
5.2.2 Milling
5.2.3 Sugarcane Juice Treatment
5.2.4 Pre-evaporation
5.2.5 Medium Preparation
5.2.6 Fermentation

5.2.7 Wine Centrifugation
5.2.8 Distillation
5.2.9 Dehydration
5.2.10 Quantitative Process Data
5.3 Waste Production and Reuse in Sugarcane Bioethanol Units
5.4 Biogas Production Within Bioethanol Units
5.4.1 Sugarcane Bagasse
5.4.2 Filter Cake
5.4.3 Vinasse
5.5 Electric Power Generation
5.6 Conclusion and Future Perspective
References
Chapter 6: Corn First-Generation Bioethanol Unities with Energy and Dried Grains with Solubles (DDGS) Production
6.1 Introduction
6.2 Corn Preparation and Pretreatment for Bioethanol Production