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001451102 001__ 1451102
001451102 003__ OCoLC
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001451102 019__ $$a1350687209
001451102 020__ $$a9783031080722$$q(electronic bk.)
001451102 020__ $$a3031080726$$q(electronic bk.)
001451102 020__ $$z9783031080715
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001451102 0247_ $$a10.1007/978-3-031-08072-2$$2doi
001451102 035__ $$aSP(OCoLC)1350617432
001451102 040__ $$aYDX$$beng$$erda$$epn$$cYDX$$dGW5XE$$dEBLCP$$dUKAHL$$dOCLCQ
001451102 049__ $$aISEA
001451102 050_4 $$aSB123.57
001451102 08204 $$a631.5/233$$223/eng/20221122
001451102 24500 $$aGenome editing :$$bcurrent technology advances and applications for crop improvement /$$cShabir Hussain Wani, Goetz Hensel, editors.
001451102 264_1 $$aCham :$$bSpringer,$$c[2022]
001451102 264_4 $$c©2022
001451102 300__ $$a1 online resource (x, 345 pages) :$$billustrations (chiefly color)
001451102 336__ $$atext$$btxt$$2rdacontent
001451102 337__ $$acomputer$$bc$$2rdamedia
001451102 338__ $$aonline resource$$bcr$$2rdacarrier
001451102 500__ $$aIncludes index.
001451102 5050_ $$aIntro -- Foreword -- Preface -- Contents -- Part I: Current Status and Challenges of Plant Genome Editing Using CRISPR/Cas Technology -- Genome Engineering as a Tool for Enhancing Crop Traits: Lessons from CRISPR/Cas9 -- 1 Introduction -- 2 Zinc-Finger Nucleases -- 3 Transcription Activator-Like Effector Nucleases -- 4 CRISPR/Cas9 System -- 5 Mechanism of CRISPR/Cas9 System -- 6 Targeted Improvement of Crop Traits Using CRISPR/Cas9-Based Genome Editing in Cereals -- 6.1 Resistance Against Bacterial Disease -- 6.2 Resistance Against Fungal Disease -- 6.3 Resistance Against Viruses
001451102 5058_ $$a6.4 Resistance and Tolerance Against Herbicides -- 6.5 Improved Quality and Yield -- 7 CRISPR/Cas9 Mediated Genome Editing in Horticultural Crops -- 7.1 Resistance Against Bacterial Disease -- 7.2 Resistance Against Fungal Disease -- 7.3 Resistance Against Viruses -- 7.4 Resistance and Tolerance Against Herbicide -- 7.5 Improved Quality and Yield -- 8 Conclusion -- References -- Vegetable Crop Improvement Through CRISPR Technology for Food Security -- 1 Introduction -- 2 Applications of Genome Editing in Improvement of Vegetable Crops -- 2.1 Qualitative Traits -- 2.1.1 Starch Content
001451102 5058_ $$a2.1.2 Pigmentation -- 2.1.3 Saturated Fatty Acid Content -- 2.1.4 Improvement of Shelf-Life and Quality -- 2.1.5 Other Qualitative Traits -- 2.2 Abiotic Stress Tolerance -- 2.2.1 Drought and Extreme Temperature -- 2.2.2 Salinity and Mineral Deficiency -- 2.3 Pest and Disease Resistance -- 2.4 Biosafety and Legal Regulations -- 2.5 Conclusion -- References -- CRISPR/Cas9-Mediated Targeted Mutagenesis in Medicinal Plants -- 1 Introduction -- 2 CRISPR/Cas9 Mechanism -- 2.1 Cleavage Activity of Cas9 -- 3 CRISPR/Cas9 Vector System for Plants -- 3.1 sgRNA Expression Cassettes
001451102 5058_ $$a3.2 Cas9 Expression Cassettes -- 4 CRISPR/dCas9 and Epigenome Editing in Plants -- 4.1 Nuclease-Dead Cas9 -- 4.2 sgRNA -- 4.3 Transcriptional Effectors -- 5 Analysis and Efficiency of Targeted Mutations -- 5.1 Reporter Genes -- 5.2 Single-Strand Conformation Polymorphism (SSCP) -- 5.3 High-Resolution Melting (HRM) -- 5.4 High-Throughput Sequencing (HTS or Deep Sequencing) -- 5.5 Sanger Sequencing -- 6 Medicinal Plants Modified Using CRISPR/Cas9 -- 6.1 Salvia militorrhiza -- 6.2 Dendrobium officinale -- 6.3 Dioscorea zingiberensis -- 7 Applications of Genome Editing in Medicinal Plants
001451102 5058_ $$a8 Conclusion -- References -- Genome Editing: A Review of the Challenges and Approaches -- 1 Introduction -- 2 Mechanisms of Repairing Double-Stranded Breaks -- 2.1 Non-Homologous End Joining (NHEJ) -- 2.2 Genome editing and Homologous Recombination -- 2.2.1 History of Genome Editing -- 2.2.2 Homologous Recombination in E. coli -- 2.2.3 HR in Saccharomyces cerevisiae -- 2.2.4 HR in Higher Organisms -- 3 Different Genome Editing Techniques -- 3.1 Meganucleases (MNs) -- 3.2 Zinc Finger Nucleases (ZFNs) -- 3.3 Transcription Activator-Like Effector Nucleases (TALENs)
001451102 506__ $$aAccess limited to authorized users.
001451102 520__ $$aOver the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
001451102 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed November 22, 2022).
001451102 650_0 $$aTransgenic plants.
001451102 650_0 $$aCrops$$xGenetic engineering.
001451102 655_0 $$aElectronic books.
001451102 7001_ $$aWani, Shabir Hussain,$$eeditor.$$1https://isni.org/isni/0000000448061021
001451102 7001_ $$aHensel, Goetz,$$eeditor.
001451102 77608 $$iPrint version: $$z3031080718$$z9783031080715$$w(OCoLC)1317836716
001451102 852__ $$bebk
001451102 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-3-031-08072-2$$zOnline Access$$91397441.1
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