Autophagy in stem cell maintenance and differentiation / Bhupendra V. Shravage, Kursad Turksen, editors.
Shravage, Bhupendra V., editor.; Turksen, Kursad, editor.
2023
QH588.S83
Available Online

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Title Autophagy in stem cell maintenance and differentiation / Bhupendra V. Shravage, Kursad Turksen, editors.
ISBN 9783031173622 (electronic bk.)
3031173627 (electronic bk.)
9783031173615
3031173619
DOI https://doi.org/10.1007/978-3-031-17362-2
Published Cham : Springer, [2023]
Copyright ©2023
Language English
Description 1 online resource (viii, 288 pages) : illustrations (some color).
Item Number 10.1007/978-3-031-17362-2 doi
Call Number QH588.S83
Dewey Decimal Classification 616.02/774
Summary This book covers a wide range of topics that illustrate the various functions of autophagy in stem cells and offers insights on the mechanisms by which autophagy can regulate stem-cell self-renewal and facilitate specific differentiation programs. Stem cells are unique cells present in most multicellular animals and are essential for their survival. They have two unique properties: the ability to self-renew and the ability to differentiate into one or more cell types. These characteristics of stem cells have found immense therapeutic potential in regenerative medicine. Autophagy is a crucial membrane trafficking pathway that is essential for maintaining cellular homeostasis that involves sequestration of non-functional proteins, protein aggregates and damaged organelles in double-membraned vesicles called autophagosomes, which are subsequently targeted to the lysosome for degradation. The primary aim of this book is to provide knowledge of recent developments in our understanding of the role of autophagy in stem cells, including germline stem cells. Autophagy is considered a promising target for many diseases. Significant efforts are being developed to identify specific modulators of autophagy, which will aid in designing combinatorial therapeutic strategies that will allow significant improvements in regenerative medicine.
Access Note Access limited to authorized users.
Source of Description Online resource; title from PDF title page (SpringerLink, viewed November 17, 2022).
Added Author Shravage, Bhupendra V., editor.
Turksen, Kursad, editor.
Series Stem cell biology and regenerative medicine ; volume 73.
Available in Other Form Print version: 9783031173615
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Record Appears in Online Resources > Ebooks
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Intro
Preface
Contents
1 Assays for Monitoring Autophagy in Stem Cells
Introduction
Autophagy Process and Mechanism
Techniques for Assessing Autophagy Orchestration
Electron Microscopy Imaging
Fluorescence Microscopy
LC3 Antibody Tagging
Fluorescent Protein Tagging of LC3
mRFP/mCherry-GFP-LC3 Tandem Reporter Constructs and Their Advancements
GFP-LC3-RFP-LC3∆G
SQSTM/p62/Ref(2)P Tagging
Evaluation of Specific Steps During Autophagy
Autophagy Visualization Dyes
Tracking Mitophagy in Stem Cells
Atg8s/LC3 and p62 Protein Expression in Stem Cells

LC3 Turnover Assays
Flow Cytometry and Fluorescence-Associated Cell Sorting (FACS)
Potential Probes for Monitoring Autophagy
Gene Expression Assays
Protein Interaction Databases
Conclusion
References
2 Autophagy in Stem Cell Maintenance and Differentiation
Introduction
Autophagy Compared to Necrosis and Apoptosis
The Autophagic Cascade
Types of Autophagy
Role of Autophagy in Stem Cell Maintenance
Role of Autophagy in Maintenance of Hematopoietic Stem Cells (HSCs)
Role of Autophagy in Maintenance of Neural Stem Cells

Role of Autophagy in Maintenance of Muscle Stem Cells
Role of Autophagy in Maintenance of Bone Marrow Derived Mesenchymal Stem Cells (BMSCs)
Role of Autophagy in Maintenance of Hepatic Progenitor Cells
Role of Autophagy in Maintenance of Intestinal Stem Cells (ISCs)
Autophagy and Stem Cell Differentiation
Role of Autophagy in the Differentiation of HSCs
Role of Autophagy in the Differentiation of NSCs
Role of Autophagy in the Differentiation of Cardiac Stem Cells
Role of Autophagy in Adipocyte Formation from MSCs
Role of Autophagy in Osteoclastogenesis

Role of Autophagy During Embryogenesis
Conclusion
References
3 Autophagy in Embryonic Stem Cells and Neural Stem Cells
Introduction
Embryonic Stem Cells
Autophagy in ESCs and During Embryogenesis: Evidence and Importance
Specific Roles of Autophagy in ESCs
Autophagy and Proteostasis in ESCs
Autophagy and Pluripotency Factor Turnover
Mitophagy in ESCs
Midbody Ring, Autophagy, and ESCs
Regulation of Autophagy in ESCs
Autophagy and Neural Stem Cells
Neural Stem Cells
Evidence for Autophagy in Neural Stem Cells

Implications of Autophagy Defects in Development and Disease
Conclusions and Outlook
References
4 Autophagy in Germline Stem Cells
Overview of Autophagy
Overview of GSCs in Model Organisms
Perturbed Autophagy Affects Fertility
Autophagy and Signaling in GSCs
Autophagy, Lipid Metabolism, and Germline Stem Cells
Autophagy and Organelle Homeostasis in GSCs
Autophagy in Programmed Cell Death and Germline Development
Conclusions
References
5 The Role of Autophagy in the Regulation of Hematopoietic Stem Cells
Introduction
Autophagy
Types of Autophagy

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