001454498 000__ 03665nam\a2200553\i\4500
001454498 001__ 1454498
001454498 003__ OCoLC
001454498 005__ 20230314003215.0
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001454498 008__ 230209s2023\\\\sz\a\\\\ob\\\\001\0\eng\d
001454498 020__ $$a9783031194856$$q(electronic bk.)
001454498 020__ $$a3031194853$$q(electronic bk.)
001454498 020__ $$z3031194845
001454498 020__ $$z9783031194849
001454498 0247_ $$a10.1007/978-3-031-19485-6$$2doi
001454498 035__ $$aSP(OCoLC)1369492843
001454498 040__ $$aGW5XE$$beng$$erda$$epn$$cGW5XE
001454498 049__ $$aISEA
001454498 050_4 $$aQH585.2
001454498 08204 $$a571.6/38078$$223/eng/20230209
001454498 1001_ $$aMani, Shalini,$$eauthor.
001454498 24510 $$aAnimal cell culture :$$bprinciples and practice /$$cShalini Mani, Manisha Singh, Anil Kumar.
001454498 264_1 $$aCham :$$bSpringer,$$c[2023]
001454498 264_4 $$c©2023
001454498 300__ $$a1 online resource (xvi, 327 pages) :$$billustrations (chiefly color).
001454498 336__ $$atext$$btxt$$2rdacontent
001454498 337__ $$acomputer$$bc$$2rdamedia
001454498 338__ $$aonline resource$$bcr$$2rdacarrier
001454498 4901_ $$aTechniques in life science and biomedicine for the non-expert
001454498 504__ $$aIncludes bibliographical references and index.
001454498 5050_ $$a1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques.
001454498 506__ $$aAccess limited to authorized users.
001454498 520__ $$aThis introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. .
001454498 588__ $$aDescription based on print version record.
001454498 650_0 $$aCell culture$$vLaboratory manuals.
001454498 655_7 $$aLaboratory manuals.$$2fast$$0(OCoLC)fst01920776
001454498 655_7 $$aLaboratory manuals.$$2lcgft
001454498 655_0 $$aElectronic books.
001454498 7001_ $$aSingh, Manisha,$$eauthor.
001454498 7001_ $$aKumar, Anil,$$eauthor.
001454498 77608 $$iPrint version:$$aMANI, SHALINI. SINGH, MANISHA. KUMAR, ANIL.$$tANIMAL CELL CULTURE.$$d[Place of publication not identified] : SPRINGER INTERNATIONAL PU, 2022$$z3031194845$$w(OCoLC)1346071312
001454498 830_0 $$aTechniques in life science and biomedicine for the non-expert.
001454498 852__ $$bebk
001454498 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-3-031-19485-6$$zOnline Access$$91397441.1
001454498 909CO $$ooai:library.usi.edu:1454498$$pGLOBAL_SET
001454498 980__ $$aBIB
001454498 980__ $$aEBOOK
001454498 982__ $$aEbook
001454498 983__ $$aOnline
001454498 994__ $$a92$$bISE