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Table of Contents
Intro
Summary
Zusammenfassung
1 Introduction
1.1 General introduction
1.2 Objectives
2 Theoretical Background
2.1 Biomass as a resource
2.2 Depolymerization of biomass
2.3 Bioconversion of biomass-derived carbon
2.4 Cis,cis-muconic acid - an important platform chemical
3 Material and Methods
3.1 Lignin and hemicellulose
3.2 Hydrothermal lignin conversion
3.3 Hydrothermal lignin conversion at miniaturized scale
3.4 Hydrothermal hemicellulose conversion
3.5 Fractionation of depolymerized hemicellulose by distillation
3.6 Distillation of lignin hydrolysate
3.7 Concentration of lignin and hemicellulose hydrolysates
3.8 Decolorization of hemicellulose hydrolysates and removal oftoxic ingredients
3.9 Microorganisms and plasmids
3.10 Media
3.11 Cultivation of Pseudomonas putida
3.12 Cultivation of Corynebacterium glutamicum strains
3.13 Cultivation of Amycolatopsis sp.
3.14 Fed-batch production of cis,cis-muconic acid
3.15 Quantification of glucose and xylose
3.16 Quantification of mono- and disaccharides
3.17 Quantification of aromatics and muconic acid derivatives
3.18 Fingerprinting of aromatics in lignin hydrolysates
3.19 Quantification of organic acids and alcohols
3.20 Quantification of cell concentration
4 Results and Discussion
4.1 Microbial production of cis,cis-muconic acid from aromatics
4.2 Cascaded production of cis,cis-muconic acid from Kraft lignin
4.3 Impact of the lignin-type on depolymerization and bioconversionefficiency
4.4 Gram-scale production of cis,cis-muconic acid from repeatedfeeding of lignin hydrolysate
4.5 The use of hemicellulose-derived sugars as co-substrate enablesan entirely lignocellulose-based process
5 Conclusion and Outlook
6 Appendix
6.1 Abbreviations.
6.2 Cultivation of P. putida MA-9 on organosolv lignin hydrolysate
6.3 Aromatic monomers obtained from multiple runs of hydrothermal
6.4 Cultivation of C. glutamicum MA-3 on hemicellulose hydrolysatein a bioreactor with addition of lignin hydrolysate
6.5 Overview of results from hydrothermal conversion of lignin andsubsequent bioconversion to muconic acids
7 References.
Summary
Zusammenfassung
1 Introduction
1.1 General introduction
1.2 Objectives
2 Theoretical Background
2.1 Biomass as a resource
2.2 Depolymerization of biomass
2.3 Bioconversion of biomass-derived carbon
2.4 Cis,cis-muconic acid - an important platform chemical
3 Material and Methods
3.1 Lignin and hemicellulose
3.2 Hydrothermal lignin conversion
3.3 Hydrothermal lignin conversion at miniaturized scale
3.4 Hydrothermal hemicellulose conversion
3.5 Fractionation of depolymerized hemicellulose by distillation
3.6 Distillation of lignin hydrolysate
3.7 Concentration of lignin and hemicellulose hydrolysates
3.8 Decolorization of hemicellulose hydrolysates and removal oftoxic ingredients
3.9 Microorganisms and plasmids
3.10 Media
3.11 Cultivation of Pseudomonas putida
3.12 Cultivation of Corynebacterium glutamicum strains
3.13 Cultivation of Amycolatopsis sp.
3.14 Fed-batch production of cis,cis-muconic acid
3.15 Quantification of glucose and xylose
3.16 Quantification of mono- and disaccharides
3.17 Quantification of aromatics and muconic acid derivatives
3.18 Fingerprinting of aromatics in lignin hydrolysates
3.19 Quantification of organic acids and alcohols
3.20 Quantification of cell concentration
4 Results and Discussion
4.1 Microbial production of cis,cis-muconic acid from aromatics
4.2 Cascaded production of cis,cis-muconic acid from Kraft lignin
4.3 Impact of the lignin-type on depolymerization and bioconversionefficiency
4.4 Gram-scale production of cis,cis-muconic acid from repeatedfeeding of lignin hydrolysate
4.5 The use of hemicellulose-derived sugars as co-substrate enablesan entirely lignocellulose-based process
5 Conclusion and Outlook
6 Appendix
6.1 Abbreviations.
6.2 Cultivation of P. putida MA-9 on organosolv lignin hydrolysate
6.3 Aromatic monomers obtained from multiple runs of hydrothermal
6.4 Cultivation of C. glutamicum MA-3 on hemicellulose hydrolysatein a bioreactor with addition of lignin hydrolysate
6.5 Overview of results from hydrothermal conversion of lignin andsubsequent bioconversion to muconic acids
7 References.