@article{1459982,
      recid = {1459982},
      author = {Mukherjee, Tapan Kumar, and Malik, Parth, and Mukherjee,  Srirupa,},
      title = {Practical approach to mammalian cell and organ culture /},
      pages = {1 online resource :},
      abstract = {This Major Reference Work offers a detailed overview of  culturing primary, secondary cell lines, tissues, and  organs. It first introduces various types of mammalian cell  cultures, infrastructure requirements for a mammalian  cell-culture laboratory. The subsequent chapters present  the detailed protocols for the isolation of mammalian  hematologic organs and cells. It also discusses various  cell-based assays for monitoring cell viability, cell  proliferation, cytotoxicity, cell senescence, and cell  death assays. In addition, the book addresses the various  problems encountered while culturing animal cells, their  possible causes, and suggested solutions, presenting  detailed protocols for isolation and primary culturing of  various mammalian cells and hematoimmunologic organs in two  dimensions. Lastly, it reviews the various applications of  animal-cell culture, stem-cell culture, and tissue and  organ culture. As such, this reference book is highly  relevant for students and professionals new to cell-culture  work as well as to those wishing to expand their skills  from cell-line cultures to primary cultures and from  conventional 2D cultures to 3D cultures.},
      url = {http://library.usi.edu/record/1459982},
      doi = {https://doi.org/10.1007/978-981-19-1731-8},
}