001459982 000__ 04144nam\a22004697i\4500 001459982 001__ 1459982 001459982 003__ OCoLC 001459982 005__ 20230330003237.0 001459982 006__ m\\\\\o\\d\\\\\\\\ 001459982 007__ cr\un\nnnunnun 001459982 008__ 230223s2022\\\\si\a\\\\o\\\\\000\0\eng\d 001459982 020__ $$a9789811917318$$q(electronic bk.) 001459982 020__ $$a9811917310$$q(electronic bk.) 001459982 020__ $$z9789811917301$$q(print) 001459982 0247_ $$a10.1007/978-981-19-1731-8$$2doi 001459982 035__ $$aSP(OCoLC)1371023462 001459982 040__ $$aGW5XE$$beng$$erda$$epn$$cGW5XE 001459982 049__ $$aISEA 001459982 050_4 $$aQH585 001459982 08204 $$a571.5/38$$223/eng/20230223 001459982 24500 $$aPractical approach to mammalian cell and organ culture /$$cedited by Tapan Kumar Mukherjee, Parth Malik, Srirupa Mukherjee. 001459982 264_1 $$aSingapore :$$bSpringer,$$c2022. 001459982 300__ $$a1 online resource :$$billustrations (some color) 001459982 336__ $$atext$$btxt$$2rdacontent 001459982 337__ $$acomputer$$bc$$2rdamedia 001459982 338__ $$aonline resource$$bcr$$2rdacarrier 001459982 5050_ $$aMammalian Cell Culture: An Overview -- Establishment of a Cell Culture Laboratory -- Mammalian Cell Culture Laboratory: Equipment and Other Materials -- Common Reagents and Medium for Mammalian Cell Culture -- Microbial Contamination of Mammalian Cell Culture -- Mammalian Cell Culture Types and Guidelines for Their Maintenance -- Isolation and Purification of Various Mammalian Cells: Single Cell Isolation -- Isolation and Primary Culture of Various Mammalian Cells -- Primary Culture of Immunological Cells -- Culture of Neuron and Glia Cells -- Culture of Continuous Cell Lines -- Stem Cell Culture and Its Applications -- Mammalian Cell Culture in Three Dimensions: Basic Guidelines -- Organ, Histotypic and Organotypic Culture, and Tissue Engineering -- Large-Scale Culture of Mammalian Cells for Various Industrial Purposes -- Experimental Mammalian Cell Culture-Based Assays -- Mammalian Cells, Tissues and Organ Culture: Applications -- Biosensors’ Utility in Mammalian Cell Culturing -- Nanomaterials: Compatibility Towards Biological Interactions -- Review on IPR and Technological Advancements in Nanotechnology for Nanomedicine -- Emerging Drug Delivery Potential of Gold and Silver Nanoparticles to Lung and Breast Cancers -- Mammalian Cells: Reliability as Model System in the Ecotoxicological Evaluation of Environmental Stressors -- Bioimaging: Usefulness in Modern Day Research -- Troubleshooting of Mammalian Cell Culture. 001459982 506__ $$aAccess limited to authorized users. 001459982 520__ $$aThis Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures. 001459982 588__ $$aTitle details screen. 001459982 650_0 $$aCell culture. 001459982 650_0 $$aOrgan culture. 001459982 655_0 $$aElectronic books. 001459982 7001_ $$aMukherjee, Tapan Kumar,$$eeditor. 001459982 7001_ $$aMalik, Parth,$$eeditor. 001459982 7001_ $$aMukherjee, Srirupa,$$eeditor. 001459982 852__ $$bebk 001459982 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-981-19-1731-8$$zOnline Access$$91397441.1 001459982 909CO $$ooai:library.usi.edu:1459982$$pGLOBAL_SET 001459982 980__ $$aBIB 001459982 980__ $$aEBOOK 001459982 982__ $$aEbook 001459982 983__ $$aOnline 001459982 994__ $$a92$$bISE