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Table of Contents
Part I - Fluorescence Spectroscopy: Basics and Advanced Approaches
Chapter 1 Choosing the right fluorescent probe
Chapter 2 Fluorescence kinetics and time-resolved measurement
Chapter 3 Quantitative Approach to Applications of Electronic Energy Transfer (EET)
Chapter 4 Single-molecule FRET: principles and analysis
Chapter 5 Principles of fluorescence correlation and dual-color cross-correlation spectroscopy
Part II - Fluorescence Microscopy: Basics and Advanced Approaches
Chapter 6 Introduction to Fluorescence Microscopy
Chapter 7 Wohland STED and RESOLFT fluorescent nanoscopy
Chapter 8 Fluorescence Correlation Spectroscopy in Space and Time
Part C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes
Chapter 9 Determination of Biomolecular Oligomerization in the Live Cell Plasma Membrane via Single-molecule Brightness and Co-localization Analysis
Chapter 10 Quantitative photoactivated localization microscopy of membrane receptor oligomers
Chapter 11 Diffusion measurements at the nanoscale with STED-FCS
Chapter 12 Single molecule microscopy methods to study mitochondrial processes
Chapter 13 Transient state (TRAST) spectroscopy and imaging exploiting the rich information source of fluorophore dark state transitions in biomolecular and cellular studies
Chapter 14 The analysis of in-membrane nanoscopic aggregation of lipids and proteins by MC-FRET
Part D - Applications of Fluorescence Spectroscopy to protein studies
Chapter 15 Single-molecule fluorescence spectroscopy of intrinsically disordered proteins
Chapter 16 Insights into the conformational dynamics of potassium channels using homo-FRET approaches
Chapter 17 Intrinsic fluorescence kinetics in proteins
Chapter 18 Dynamics and hydration of proteins viewed by fluorescence methods: investigations famor protein engineering and synthetic biology.
Chapter 1 Choosing the right fluorescent probe
Chapter 2 Fluorescence kinetics and time-resolved measurement
Chapter 3 Quantitative Approach to Applications of Electronic Energy Transfer (EET)
Chapter 4 Single-molecule FRET: principles and analysis
Chapter 5 Principles of fluorescence correlation and dual-color cross-correlation spectroscopy
Part II - Fluorescence Microscopy: Basics and Advanced Approaches
Chapter 6 Introduction to Fluorescence Microscopy
Chapter 7 Wohland STED and RESOLFT fluorescent nanoscopy
Chapter 8 Fluorescence Correlation Spectroscopy in Space and Time
Part C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes
Chapter 9 Determination of Biomolecular Oligomerization in the Live Cell Plasma Membrane via Single-molecule Brightness and Co-localization Analysis
Chapter 10 Quantitative photoactivated localization microscopy of membrane receptor oligomers
Chapter 11 Diffusion measurements at the nanoscale with STED-FCS
Chapter 12 Single molecule microscopy methods to study mitochondrial processes
Chapter 13 Transient state (TRAST) spectroscopy and imaging exploiting the rich information source of fluorophore dark state transitions in biomolecular and cellular studies
Chapter 14 The analysis of in-membrane nanoscopic aggregation of lipids and proteins by MC-FRET
Part D - Applications of Fluorescence Spectroscopy to protein studies
Chapter 15 Single-molecule fluorescence spectroscopy of intrinsically disordered proteins
Chapter 16 Insights into the conformational dynamics of potassium channels using homo-FRET approaches
Chapter 17 Intrinsic fluorescence kinetics in proteins
Chapter 18 Dynamics and hydration of proteins viewed by fluorescence methods: investigations famor protein engineering and synthetic biology.