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001468367 001__ 1468367
001468367 003__ OCoLC
001468367 005__ 20230707003248.0
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001468367 008__ 230602s2023\\\\si\\\\\\ob\\\\000\0\eng\d
001468367 019__ $$a1381093398
001468367 020__ $$a9789819906918$$q(electronic bk.)
001468367 020__ $$a9819906911$$q(electronic bk.)
001468367 020__ $$z9819906903
001468367 020__ $$z9789819906901
001468367 0247_ $$a10.1007/978-981-99-0691-8$$2doi
001468367 035__ $$aSP(OCoLC)1380844388
001468367 040__ $$aYDX$$beng$$cYDX$$dGW5XE$$dEBLCP
001468367 049__ $$aISEA
001468367 050_4 $$aRC157
001468367 08204 $$a616.9/3620072$$223/eng/20230615
001468367 1001_ $$aKulkeaw, Kasem.
001468367 24510 $$aEmergence of in vitro 3D systems to model human malaria /$$cKasem Kulkeaw.
001468367 260__ $$aSingapore :$$bSpringer,$$c2023.
001468367 300__ $$a1 online resource
001468367 504__ $$aIncludes bibliographical references.
001468367 5050_ $$aIntro -- Preface -- Contents -- About the Author -- 1: Introduction to Malaria Biology in a Liver -- 1.1 Global Impact of Malaria in the Past 10 Years -- 1.2 Liver-Stage Malaria -- 1.2.1 Life Cycle of Plasmodium Species -- 1.2.1.1 Liver-Stage Malaria -- 1.2.1.2 Erythrocyte Stage Malaria -- 1.2.1.3 Mosquito Stage -- 1.3 Mechanism of Parasite Invasion into a Liver -- 1.3.1 Liver Structure and Composition -- 1.3.2 Hypothesis of the Sporozoite Invasion Mechanism -- 1.3.2.1 Motility of Sporozoites -- 1.3.2.2 Passage of Sporozoites from the Blood Circulation Through the Liver Sinusoid Endothelium
001468367 5058_ $$a1.3.2.3 Host Response to Hepatocyte Invading Parasite -- 1.4 Hypnozoite -- 1.5 Drug-Targeting Liver-Stage Malaria -- 1.5.1 Intra-erythrocytic Parasite -- 1.5.2 Intra-hepatic Parasite -- References -- 2: Modeling a Liver-Stage Malaria -- 2.1 Necessity of the In Vitro Models for Drug Discovery and Pre-clinical Study -- 2.2 In Vitro 2D Models -- 2.2.1 Human Hepatoma Lines -- 2.2.2 Primary Human Hepatocytes (PHH) -- 2.2.3 Human Immortalized Hepatic Cells -- 2.2.4 Human Hepatocyte-like Cells Derived from Pluripotent Stem Cells -- 2.2.4.1 Single Cell Type -- 2.2.4.2 Co-culture -- 2.3 Animal Models
001468367 5058_ $$a2.3.1 Rodent Malaria -- 2.3.2 Humanized Mice -- 2.4 The Use of the 2D Cell Culture for Antimalarial Drug Assay -- References -- 3: Molecular and Cellular Basis of Liver Organoid -- 3.1 Basis of Liver Generation and Regeneration -- 3.1.1 Development of a Liver -- 3.1.2 Cell Signaling in Hepatogenesis -- 3.1.3 Liver Regeneration -- 3.2 Biology of Liver Organoid -- 3.2.1 Definition and Characteristics of Organoid -- 3.2.2 Sources of Stem Cells for Liver Organoid Generation -- 3.2.2.1 Liver Organoid from Adult Stem Cells in a Primary Tissue -- 3.2.2.2 Liver Organoid from Pluripotent Stem Cells
001468367 5058_ $$a3.3 Types of Liver Organoid -- 3.3.1 Single Cell Type-Derived Hepatic Organoids -- 3.3.2 Unknown Cell Type -- 3.3.3 Multiple Cell Types for Heterogenous Organoids -- References -- 4: Generation of Liver Organoid -- 4.1 Culture of Human iPSCs (Si-Tayeb et al. 2010) -- 4.1.1 Preparation of the Matrigel-Coated Well -- 4.1.1.1 Preparation of Aliquots of Matrigel -- 4.1.1.2 Coating of a Cell Culture Well Plate -- 4.1.2 Preparation of the Culture Medium for Human iPSCs -- 4.1.2.1 Preparation of 20x E8 Supplement -- 4.1.2.2 Preparation of a Complete Medium for Human iPSCs
001468367 5058_ $$a4.1.3 Thawing the Frozen iPSCs -- 4.1.4 Cell Passaging -- 4.1.5 Evaluation of Cell Pluripotency -- 4.1.5.1 Morphology of the Undifferentiated iPSCs -- 4.1.5.2 Detection of Cell Surface Markers and Pluripotent Proteins -- 4.2 Generation of Hepatic Endoderms from Human iPSCs (Si-Tayeb et al. 2010) -- 4.2.1 Step 1: Monolayers of Human iPSCs (Day 5-Day 0) -- 4.2.2 Step 2: Endoderm Induction (Day 0-5) -- 4.2.3 Step 3: Specification of Hepatic Endoderm (Day 6-10) -- 4.2.4 Step 4: Differentiation of the Hepatic Endoderm into Hepatoblasts (Day 11-15) -- 4.2.5 Step 4: Hepatocyte Maturation (Day 16-20)
001468367 506__ $$aAccess limited to authorized users.
001468367 520__ $$aThis book illustrates the importance and advances of the disease model for malaria, a globally affected public health problem. This book provides comprehensive information on the malaria biology in a liver and all in vitro platforms for liver-stage malaria, including principles, protocols, applications for disease modeling and drug screening, and their limitations. The initial chapter describes the basis of stem cells in liver generation during development and in adults. The subsequent chapters highlight recent and emerging advances in liver organoid and liver-on-a-chip in modeling malaria. The book presents current protocols and methods to generate liver organoid and liver-on-a-chip together with their advantages and limitations. Toward the end, the book examines the humanized mouse model of liver-stage malaria using ectopic artificial livers regarding novel readout modalities. The recent advancement and challenges in combining liver-on-a-chip technology with biosensors are highlighted for assessing hepatocyte development viability and functions. The book elucidates the potential of these 3D models to understand the biological complexity of cellular and molecular mechanisms involved in Plasmodium development in the liver, toolboxes to investigate parasite deployment in the 3D models, and to implement in drug discovery. Finally, the book discusses the future directions and challenges in the applications of liver organoids and liver on-chip in the biology of live-stage malaria. This book is helpful for researchers and scientists in the field of parasitology, cell biology, tissue engineering, and pharmacology.
001468367 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed June 15, 2023).
001468367 650_0 $$aMalaria$$xResearch.
001468367 650_0 $$aMalaria$$xSimulation methods.
001468367 655_0 $$aElectronic books.
001468367 77608 $$iPrint version: $$z9819906903$$z9789819906901$$w(OCoLC)1366123852
001468367 852__ $$bebk
001468367 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-981-99-0691-8$$zOnline Access$$91397441.1
001468367 909CO $$ooai:library.usi.edu:1468367$$pGLOBAL_SET
001468367 980__ $$aBIB
001468367 980__ $$aEBOOK
001468367 982__ $$aEbook
001468367 983__ $$aOnline
001468367 994__ $$a92$$bISE