Methods in cancer stem cell biology / Said M. Afify, Masaharu Seno.
Afify, Said M.; Seno, Masaharu.
2023
RC269
Available Online

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Title Methods in cancer stem cell biology / Said M. Afify, Masaharu Seno.
Author Afify, Said M.
ISBN 9789819913312 (electronic bk.)
9819913314 (electronic bk.)
9819913306
9789819913305
DOI https://doi.org/10.1007/978-981-99-1331-2
Publication Details Singapore : Springer, 2023.
Language English
Description 1 online resource (251 p.)
Item Number 10.1007/978-981-99-1331-2 doi
Call Number RC269
Dewey Decimal Classification 616.994
Summary This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters if this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings. .
Bibliography, etc. Note Includes bibliographical references.
Access Note Access limited to authorized users.
Source of Description Online resource; title from PDF title page (SpringerLink, viewed June 6, 2023).
Added Author Seno, Masaharu.
Available in Other Form Methods in Cancer Stem Cell Biology
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Record Appears in Online Resources > Ebooks
All Resources
1. On the origin of cancer
2. Culture of Cells: Basic Principles
3. Stem cell culture from embryos
4. Reprogramming of normal cells
5. Maintenance of human Pluripotent Stem Cells
6. Identification of cancer stem cells by different molecular markers
7. Enrichment of Cancer Stem Cell from malignant tumor
8. Isolation of single clonal cell from primary cultured cells and establishment of a cancer stem cell line
9. Artificial Generation of Cancer Stem Cells from Normal Stem Cells
10. Quick method to assess non-mutagenic carcinogens with iPS cells
11. Self-renewal potential of caner stem cells
12. Differentiation potential of cancer stem cells in vitro
13. Tumor Angiogenesis by cancer stem cells in vivo
14. Invasion and Metastatic potential of cancer stem cells in vitro
15. Metastatic potential of cancer stem cells In Vivo- 16. Anchorage-independent cell growth assay for Cancer Stem Cells: Tumorigenic assay in vitro
17. Tumorigenic potential of cancer stem cells in vivo
18. Development of immunoliposomes using monoclonal antibodies targeting cancer stem cells
19. In-vitro evaluation of anti-cancer stem cell drugs
20. In vitro Tumoroid Model Using Cancer Stem Cells.

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