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Table of Contents
Intro
Preface
References
Contents
About the Editor
Chapter 1: What Is the CRISPR System and How It Is Used?
1 History
2 Conceptual Aspects
3 Molecular Mechanisms of the CRISPR-Cas System
4 The Versatile CRISPR Toolbox
References
Chapter 2: Modern Approaches to Mouse Genome Editing Using the CRISPR-Cas Toolbox and Their Applications in Functional Genomic...
1 Introduction
2 CRISPR-Cas Systems: An Expanding Toolkit for Mouse Genome Editing
3 Delivery of the CRISPR-Cas Toolbox
4 Enhancement of HDR vs. NHEJ
5 Generating Mouse Models of Human Disease
6 Humanised Mouse Models of Disease
7 Testing of Gene Therapies in Mouse Models of Human Disease
8 Prime Editing and Transcriptional Regulation Using CRISPR-Cas
9 Genomic Safe-Harbour Loci as Sites for Stable Integration and Expression
10 Uses of CRISPR-Cas in Mice for Non-clinical Purposes
11 CRISPR-Cas-Editing of Other Mammalian Genomes
References
Chapter 3: Long Non-coding RNAs and CRISPR-Cas Edition in Tumorigenesis
1 Long Non-coding RNAs: Biogenesis and Mechanisms of Action
2 The Role of Long Non-coding RNAs in Cancer
3 CRISPR-Cas Approaches for lncRNAs Editing in Cancer
4 Innovative Approaches for lncRNAs: Epigenome Editing and Imaging CRISPR
5 Conclusions and Perspectives
References
Chapter 4: The CRISPR/Cas System in Human Cancer
1 Impact of CRISPR/Cas
2 CRISPR Screening In Vitro
2.1 CRISPR Library
2.2 Transduction
2.3 Selective Pressure
2.3.1 Negative Selection
2.3.2 Positive Selection
2.3.3 FACS-Based Selection
2.3.4 Combinatorial Screen
3 CRISPR Screening In Vivo
4 CRISPRi and CRISPRa in Cancer
5 Base and Prime Editors: The Second Step into the Revolution
6 In Vivo Modeling Cancer with CRISPR
References
Chapter 5: Using CRISPR/Cas9 to Edit a Thyroid Cancer Cell Line
1 Introduction
2 Targeting MAPK Thyroid Cancer Genes
3 Targeting Noncoding RNA Genes (microRNA and lncRNA)
4 Final Considerations
References
Chapter 6: Genome Editing for Engineering the Next Generation of Advanced Immune Cell Therapies
1 Introduction
2 Exploring Genome Editing Tools for Advanced CAR T-Cell Manufacturing
3 Genome Editing for Increased Efficiency of Advanced T-Cell-Based Immunotherapies
3.1 Disrupting IC Inhibitors Through Genome Editing
3.2 Targeting Other Regulators of Immune Activity to Enhance the Safety and Therapeutic Efficacy of Advanced T-Cell-Based Immu...
4 Genome Editing Strategies to Make an Allogeneic CAR T-Cell Therapy Feasible
5 Genome Editing for Generating Fratricide-Resistant Engineered T Cells
6 Conclusion
References
Chapter 7: CRISPR Genome Editing and the Study of Chagas Disease
References
Chapter 8: Genome Editing Tools for Lysosomal Storage Disorders
1 Lysosomes: A Brief Overview
2 Lysosomal Storage Disorders
3 Genome Editing Technologies.
Preface
References
Contents
About the Editor
Chapter 1: What Is the CRISPR System and How It Is Used?
1 History
2 Conceptual Aspects
3 Molecular Mechanisms of the CRISPR-Cas System
4 The Versatile CRISPR Toolbox
References
Chapter 2: Modern Approaches to Mouse Genome Editing Using the CRISPR-Cas Toolbox and Their Applications in Functional Genomic...
1 Introduction
2 CRISPR-Cas Systems: An Expanding Toolkit for Mouse Genome Editing
3 Delivery of the CRISPR-Cas Toolbox
4 Enhancement of HDR vs. NHEJ
5 Generating Mouse Models of Human Disease
6 Humanised Mouse Models of Disease
7 Testing of Gene Therapies in Mouse Models of Human Disease
8 Prime Editing and Transcriptional Regulation Using CRISPR-Cas
9 Genomic Safe-Harbour Loci as Sites for Stable Integration and Expression
10 Uses of CRISPR-Cas in Mice for Non-clinical Purposes
11 CRISPR-Cas-Editing of Other Mammalian Genomes
References
Chapter 3: Long Non-coding RNAs and CRISPR-Cas Edition in Tumorigenesis
1 Long Non-coding RNAs: Biogenesis and Mechanisms of Action
2 The Role of Long Non-coding RNAs in Cancer
3 CRISPR-Cas Approaches for lncRNAs Editing in Cancer
4 Innovative Approaches for lncRNAs: Epigenome Editing and Imaging CRISPR
5 Conclusions and Perspectives
References
Chapter 4: The CRISPR/Cas System in Human Cancer
1 Impact of CRISPR/Cas
2 CRISPR Screening In Vitro
2.1 CRISPR Library
2.2 Transduction
2.3 Selective Pressure
2.3.1 Negative Selection
2.3.2 Positive Selection
2.3.3 FACS-Based Selection
2.3.4 Combinatorial Screen
3 CRISPR Screening In Vivo
4 CRISPRi and CRISPRa in Cancer
5 Base and Prime Editors: The Second Step into the Revolution
6 In Vivo Modeling Cancer with CRISPR
References
Chapter 5: Using CRISPR/Cas9 to Edit a Thyroid Cancer Cell Line
1 Introduction
2 Targeting MAPK Thyroid Cancer Genes
3 Targeting Noncoding RNA Genes (microRNA and lncRNA)
4 Final Considerations
References
Chapter 6: Genome Editing for Engineering the Next Generation of Advanced Immune Cell Therapies
1 Introduction
2 Exploring Genome Editing Tools for Advanced CAR T-Cell Manufacturing
3 Genome Editing for Increased Efficiency of Advanced T-Cell-Based Immunotherapies
3.1 Disrupting IC Inhibitors Through Genome Editing
3.2 Targeting Other Regulators of Immune Activity to Enhance the Safety and Therapeutic Efficacy of Advanced T-Cell-Based Immu...
4 Genome Editing Strategies to Make an Allogeneic CAR T-Cell Therapy Feasible
5 Genome Editing for Generating Fratricide-Resistant Engineered T Cells
6 Conclusion
References
Chapter 7: CRISPR Genome Editing and the Study of Chagas Disease
References
Chapter 8: Genome Editing Tools for Lysosomal Storage Disorders
1 Lysosomes: A Brief Overview
2 Lysosomal Storage Disorders
3 Genome Editing Technologies.