2.3 Affinity Purification of rhMEF2C Fusion Protein
2.4 SDS-PAGE and Immunoblotting
2.5 Circular Dichroism Spectroscopy
2.6 Cell Culture
2.7 Protein Transduction, Immunocytochemistry, and Microscopy
2.8 In Vitro Scratch Migration Assay
2.9 Chicken Chorioallantoic Membrane (CAM) Assay
2.10 [alpha]-MHC Reporter Assay
2.11 Flow Cytometry
2.12 Statistical Analysis
3 Results and Discussion
3.1 Codon Optimization Eliminates Rare Codons in Human MEF2C Gene as Per in Silico Analysis to Enable Enhanced Heterologous Expression

Intro
Foreword
Preface
About This Book
About IIT Guwahati
North East Research Conclave-2022: Toward Sustainable Science and Technology
From the Desk of Chairman of Technical Committee of NERC 2022
Contents
About the Editors
Cell and Molecular Biology
Generation of Recombinant Version of a Bioactive Human MEF2C Transcription Factor from E. coli
1 Introduction
2 Materials and Methods
2.1 Construction of Recombinant MEF2C Expression Plasmids Based on pET28a(+)
2.2 Expression Analysis of rhMEF2C Fusion Proteins

Identification of Candidate RNA Binding Proteins Associated with RNPS1 3'UTR
1 Introduction
2 Materials and Methods
2.1 Construction of Plasmids
2.2 Transformation of Recombinant Vector
2.3 Cell Culture
2.4 Generation of Stable Cells
2.5 RNA Isolation and cDNA Synthesis
2.6 Quantitative Real-Time PCR
2.7 Preparation of Cell Lysates
2.8 RNP Immunoprecipitation
2.9 Silver Staining
2.10 Mass Spectrometry
2.11 RBP Binding Site Prediction
2.12 Statistical Analysis
3 Results
3.1 Construction of Double Stable Cells for Interactome Capture of RNPS1 3'UTR