001480697 000__ 04830cam\\22005057i\4500 001480697 001__ 1480697 001480697 003__ OCoLC 001480697 005__ 20231031003305.0 001480697 006__ m\\\\\o\\d\\\\\\\\ 001480697 007__ cr\cn\nnnunnun 001480697 008__ 231010s2023\\\\gw\\\\\\o\\\\\000\0\eng\d 001480697 019__ $$a1401648331$$a1405397746 001480697 020__ $$a9783662672730$$q(electronic bk.) 001480697 020__ $$a3662672731$$q(electronic bk.) 001480697 020__ $$z9783662672723 001480697 0247_ $$a10.1007/978-3-662-67273-0$$2doi 001480697 035__ $$aSP(OCoLC)1402177323 001480697 040__ $$aGW5XE$$beng$$erda$$epn$$cGW5XE$$dOCLKB$$dSFB 001480697 049__ $$aISEA 001480697 050_4 $$aSB269 001480697 08204 $$a633.7/352$$223/eng/20231010 001480697 24500 $$aMutation breeding in coffee with special reference to leaf rust :$$bprotocols /$$cIvan L.W. Ingelbrecht, Maria do Céu Lavado da Silva, Joanna Jankowicz-Cieslak, editors. 001480697 264_1 $$aBerlin, Germany :$$bSpringer,$$c2023. 001480697 300__ $$a1 online resource (xx, 314 pages) :$$billustrations (some color) 001480697 336__ $$atext$$btxt$$2rdacontent 001480697 337__ $$acomputer$$bc$$2rdamedia 001480697 338__ $$aonline resource$$bcr$$2rdacarrier 001480697 5050_ $$aIntroduction coffee breeding and challenges -- Choice of materials for mutation induction in arabica coffee -- Improved in-vitro establishment and germination of Coffea arabica seed -- Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents -- Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) -- Chemical mutagenesis of Coffea arabica mature seed using EMS -- Physical mutagenesis of coffee seeds -- In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis -- Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings -- Protocol on mutation induction in coffee using in vitro tissue cultures -- Screening for resistance to coffee leaf rust -- Protocol to send samples of coffee leaf rust to CIFC -- Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions -- Development of a PCR-Based Molecular Detection -- Technique for the Early Diagnosis of Coffee Leaf -- Rust Caused by Hemileia vastatrix -- Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee. 001480697 5060_ $$aOpen access.$$5GW5XE 001480697 520__ $$aThis open-access book presents essential concepts and new, illustrated methods for mutation-assisted breeding of Coffea arabica L. (Arabica), one of the world's most important cash crops and beverages. Arabica coffee accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book covers practical methods to enhance genetic diversity in Arabica coffee through induced mutagenesis and for screening for resistance to CLR. Current breeding approaches, challenges, and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Based on latest advances in science and technology, this book includes novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter provides step-by-step illustrated methods supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications. 001480697 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed October 10, 2023). 001480697 650_0 $$aCoffee$$xBreeding.$$0(DLC)sh 85027699 001480697 650_0 $$aCoffee$$xGenetics.$$0(DLC)sh 85027699 001480697 655_0 $$aElectronic books. 001480697 7001_ $$aIngelbrecht, Ivan L. W.$$eeditor. 001480697 7001_ $$aSilva, Maria do Céu Lavado da,$$eeditor.$$0(orcid)0000-0002-1628-7128$$1https://orcid.org/0000-0002-1628-7128 001480697 7001_ $$aJankowicz-Cieslak, Joanna,$$eeditor.$$0(orcid)0000-0002-9249-4700$$1https://orcid.org/0000-0002-9249-4700 001480697 852__ $$bebk 001480697 85640 $$3Springer Nature$$uhttps://link.springer.com/10.1007/978-3-662-67273-0$$zOnline Access$$91397441.2 001480697 909CO $$ooai:library.usi.edu:1480697$$pGLOBAL_SET 001480697 980__ $$aBIB 001480697 980__ $$aEBOOK 001480697 982__ $$aEbook 001480697 983__ $$aOnline 001480697 994__ $$a92$$bISE