Intro
Preface
Contents
About the Editors
Abbreviations
Chapter 1: Introduction of Nanozymes
1.1 Nanozyme: Discovery and Definition
1.2 Characteristics of Nanozymes
1.3 Progress of Nanozyme Research
1.4 Outlook
References
Chapter 2: Regulating Nanozymes for Bioanalysis
2.1 Classification and Types of Nanozymes
2.2 Regulation of the Catalytic Activity and Selectivity of Nanozymes
2.2.1 Regulation of the Catalytic Activity
2.2.1.1 Size Engineering
2.2.1.2 Morphology Engineering
2.2.1.3 Composition Engineering
2.2.1.4 Structural Engineering

2.2.1.5 Others
2.2.2 Regulation of the Selectivity
2.2.2.1 Molecular Imprinting
2.2.2.2 Structural Engineering
2.3 Applications of Nanozymes in Bioanalysis
2.3.1 Detection of Ions
2.3.2 Detection of Small Biomolecules
2.3.3 Detection of Nucleic Acids
2.3.4 Detection of Proteins
2.3.5 Detection of Cells
2.4 Conclusion and Perspectives
References
Chapter 3: Nanozymes for In Vitro Analysis
3.1 Methodologies
3.1.1 Colorimetry
3.1.2 Electrochemistry
3.1.3 Chemiluminescence
3.1.4 Electrochemiluminescence
3.1.5 Fluorescence

3.1.6 Surface-Enhanced Raman Scattering
3.2 Nanozymes for Small Molecule Sensing
3.2.1 H2O2
3.2.2 Glucose
3.2.3 Ascorbic Acid
3.2.4 Glutathione and Thioalcohol
3.3 Nanozymes for Biomacromolecule Sensing
3.3.1 Antigen
3.3.2 RNA and DNA
3.3.3 Protein
3.4 Nanozymes for Bacteria and Viruses Sensing
3.5 Nanozymes for Cancer Cells and Exosomes Sensing
3.6 Nanozymes for Pesticides and Metal Ions Sensing
3.7 Summary and Perspectives
References
Chapter 4: Nanozymes for Live Assays
4.1 Magnetic Resonance Imaging
4.2 Fluorescence Imaging

4.3 Photoacoustic Imaging
4.4 Multimodal Imaging
4.5 Online Measurement Based on Microdialysis
4.6 Other Methods
4.7 Summary and Perspectives
References
Chapter 5: Nanozymes for Antioxidant Therapy
5.1 Types and Preparation of Antioxidant Nanozymes
5.1.1 Elemental Non-metal Nanozymes
5.1.2 Elemental Metal Nanozymes
5.1.3 Metal Compound Nanozymes
5.1.4 Metal-Organic Framework Nanozymes
5.1.5 Single-Atom Nanozymes
5.1.6 Composite Nanozymes
5.2 Catalytic Mechanism of Antioxidant Nanozymes and Rational Design for Modulating Antioxidant Activity

5.2.1 Catalytic Mechanism of Antioxidant Nanozymes
5.2.1.1 SOD Mimicking Activity
5.2.1.2 CAT Mimicking Activity
5.2.1.3 POD Mimicking Activity
5.2.1.4 GPx Mimicking Activity
5.2.1.5 Other Antioxidant Mechanisms
5.2.1.6 Multiple Enzymes Mimicking Activity
5.2.2 Rational Design of Antioxidant Nanozymes for Modulating Antioxidant Activity
5.2.2.1 Size Modulation
5.2.2.2 Shape Modulation
5.2.2.3 Chemical Doping
5.2.2.4 Surface Modifications
5.2.2.5 Composition
5.3 Application of Antioxidant Nanozymes