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001482551 001__ 1482551
001482551 003__ OCoLC
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001482551 020__ $$a9783031387319
001482551 020__ $$a3031387317
001482551 0247_ $$a10.1007/978-3-031-38731-9$$2doi
001482551 035__ $$aSP(OCoLC)1405368533
001482551 040__ $$aEBLCP$$beng$$cEBLCP$$dGW5XE$$dEBLCP$$dYDX$$dOCLCF$$dOCLCO
001482551 049__ $$aISEA
001482551 050_4 $$aRS199.5$$b.L36 2023
001482551 08204 $$a572/.65$$223/eng/20231025
001482551 1001_ $$aLangel, Ülo,$$eauthor.
001482551 24510 $$aCPP, cell-penetrating peptides$$h[electronic resource] /$$cÜlo Langel.
001482551 250__ $$aSecond edition.
001482551 260__ $$aCham, Switzerland :$$bSpringer,$$c[2023]
001482551 300__ $$a1 online resource (xxi, 556) ;$$billustrations
001482551 500__ $$aDescription based upon print version of record.
001482551 500__ $$a10.4.1 Applications of Fluorophores in Imaging
001482551 504__ $$aIncludes bibliographical references and index.
001482551 5050_ $$aIntro -- Acknowledgment -- Contents -- Abbreviations -- About the Author -- List of Figures -- Contents -- 1 Introduction -- 1.1 Introduction to the Second Edition, 2024 -- 1.2 Introduction to First Edition -- References -- 2 Classes and Applications of Cell-Penetrating Peptides -- Abstract -- 2.1 Protein Derived Versus Designed -- 2.2 Classification by Physico-Chemical Properties Versus Structural Properties -- 2.3 Predicted Versus Random -- 2.4 Linear Versus Modified -- 2.4.1 Linear -- 2.4.2 Stabilized -- 2.5 Protein Mimics Versus Cargo Delivery Vectors -- 2.5.1 Protein-Mimicking CPPs
001482551 5058_ $$a2.5.2 CPPs for Cargo Delivery -- 2.6 Nonspecific Versus Targeted -- 2.6.1 Affinity Targeting: Homing, Ligand Based Targeting -- 2.6.2 Prodrug Approach, aCPP -- 2.7 Classification by Uptake Mechanisms -- 2.7.1 Direct Translocators -- 2.7.2 Endocytosis Enhancers -- 2.8 Non-toxic Versus Antimicrobial -- 2.8.1 Non-toxic -- 2.8.2 Antimicrobial -- References -- 3 Methods for CPP Selection, Prediction and in Silico Analysis -- Abstract -- 3.1 Selection -- 3.2 Prediction -- 3.3 Cargo Delivery Vectors -- References -- 4 Labeling of CPPs -- Abstract -- 4.1 Radioactive CPP Labelling
001482551 5058_ $$a4.2 Fluorescent Labelling of CPPs -- 4.3 Quantum Dots -- 4.4 Fluorescence Quenching -- 4.5 Luciferin-CPP Conjugates -- References -- 5 Methods for CPP Functionalization with Oligonucleotides -- Abstract -- 5.1 Antisense -- 5.1.1 Antisense Delivery -- 5.1.2 Splicing Redirection Assay -- 5.1.3 MicroRNAs and Anti-MicroRNAs -- 5.1.4 Decoy Oligonucleotides -- 5.1.5 Live Cell Genomics -- 5.2 siRNA Delivery Methods -- References -- 6 Additional Gene Therapeutic Platforms -- Abstract -- 6.1 Plasmid Delivery -- 6.2 Cellular Reprogramming -- 6.3 Genome Editing -- References
001482551 5058_ $$a7 Protein Delivery and Mimicry -- Abstract -- 7.1 Fusion Proteins -- 7.2 Chemical Conjugation of CPPs to Proteins -- 7.3 Biotin Conjugations -- 7.4 Complexation of Proteins and Peptides -- 7.5 Targeting Proteins with CPPs -- 7.6 Protein Mimicry and PPI -- 7.6.1 Delivery of Protein Mimics as Cargos by CPPs -- 7.6.2 Protein Mimics with CPP Properties -- References -- 8 General Targeting Strategies -- Abstract -- 8.1 "Addressing" -- 8.2 Prodrug Strategies -- References -- 9 Targeting Specific Barriers -- Abstract -- 9.1 Targeting Intracellular Organelles with CPPs
001482551 5058_ $$a9.1.1 Nuclear and Nucleolar Targeting -- 9.1.2 Lysosomal Targeting -- 9.1.3 Mitochondrial Targeting -- 9.1.4 Golgi and Endoplasmic Reticulum Targeting -- 9.2 Targeting Biological Tissue Barriers -- 9.2.1 Oral Administration -- 9.2.2 Inhalation Delivery -- 9.2.3 Intranasal Delivery -- 9.2.4 Transdermal Delivery -- 9.2.5 Delivery Across Blood-brain-Barrier, BBB -- 9.3 Targeting Plants -- References -- 10 Methods for Molecular Imaging, Detection and Visualization of CPPs -- Abstract -- 10.1 HPLC Analysis -- 10.2 Mass-Spectrometric Methods -- 10.3 Radionuclide Imaging -- 10.4 Optical Imaging
001482551 506__ $$aAccess limited to authorized users.
001482551 520__ $$aIn this book, a summary and update of the most important areas of cell-penetrating peptides (CPP) research are presented, while raising relevant questions for further development. The CPP sequences are presented and discussed throughout the book. The methods for testing CPP mechanisms are discussed in detail. Various approaches for the testing of endocytotic pathways of CPP uptake are also described. Different CPP uptake experiments are compared since it is becoming clear that it is often best to apply several methods in a complementary manner in order to most comprehensively evaluate CPP uptake mechanisms due to the complexity of these processes. A brief summary of functionality issues of CPPs, both in vitro and in vivo, is discussed. Therapeutic potential of CPPs and commercial developments are discussed. The present, second edition of this book is the updated and expanded version of the first edition, published in 2019. The development of the field of cell-penetrating peptides in these five years has been obvious and exciting. This second edition of the book has been partly reorganized and comprehensively expanded with the exciting research in 2019-2023. Around 2500 novel scientific articles have become available, most of them are reviewed in the second edition. Additional rapidly growing areas of high impact presented in this second edition are therapeutic developments (Chapter 16) and delivery of oligonucleotides and proteins/peptides (Chapters 5 and 6) including novel reports on genome editing with CPP assistance. Also, several additional examples are available now on clinical trials using CPPs (Chapter 15). The book is written for researchers and students in the field.
001482551 650_6 $$aPeptides.
001482551 650_6 $$aBiochimie.
001482551 650_6 $$aMédecine.
001482551 650_6 $$aVecteurs de médicaments.
001482551 650_0 $$aPeptides.$$0(DLC)sh 85004484
001482551 650_0 $$aBiochemistry.
001482551 650_0 $$aMedicine.$$0(DLC)sh 85000963
001482551 650_0 $$aDrug carriers (Pharmacy)
001482551 655_0 $$aElectronic books.
001482551 77608 $$iPrint version:$$aLangel, Ülo$$tCPP, Cell-Penetrating Peptides$$dCham : Springer International Publishing AG,c2023$$z9783031387302
001482551 852__ $$bebk
001482551 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-3-031-38731-9$$zOnline Access$$91397441.1
001482551 909CO $$ooai:library.usi.edu:1482551$$pGLOBAL_SET
001482551 980__ $$aBIB
001482551 980__ $$aEBOOK
001482551 982__ $$aEbook
001482551 983__ $$aOnline
001482551 994__ $$a92$$bISE