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Title
Cell-free production : system development / Yuan Lu, Michael C. Jewett, editors.
ISBN
9783031430251
3031430255
9783031430244
Published
Cham, Switzerland : Springer, [2023]
Language
English
Description
1 online resource (ix, 161 pages)
Other Standard Identifiers
10.1007/978-3-031-43025-1 doi
Call Number
R856 C45 2023
Dewey Decimal Classification
610.28
Summary
This book reviews the development of cell-free production platforms and offers an authoritative perspective of the latest advances and methodologies in cell-free production systems. Readers will discover the biomanufacturing potential of in vitro biotransformation (ivBT) employing purified cascade multi-enzymes, the development of hydrogel-based multi-enzymatic systems for biosynthesis, and novel insights into the optimization of biocatalytic processes. Additionally, the book explores the cell-free production and regeneration of cofactors, shedding light on strategies to enhance the efficiency and sustainability of cellular processes. In this book, particular attention is given to the progress of cell-free in vitro evolution techniques for optimizing enzyme performance, and the book also presents the integration of rapid and finely-tuned expression systems for deployable sensing applications, revolutionizing the field of biosensing. The synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes are explored, providing a deep understanding of cellular function at the molecular level. Lastly, the book covers compartmentalized cell-free expression systems for building synthetic cells, showcasing the potential for constructing artificial cellular systems with unique functionalities. Given its breadth, this book appeals to academics, researchers, and professionals interested in the forefront of biotechnology, and together with the companion volume Cell-free Macromolecular Synthesis, both books highlight the research progresses on the basic and applied research of cell-free production systems in the last few years, being invaluable resources in the field. Chapter Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Access Note
Access limited to authorized users.
Source of Description
Online resource; title from PDF title page (SpringerLink, viewed October 24, 2023).
Series
Advances in biochemical engineering/biotechnology ; 186.
Cell-free biomanufacturing by purified cascade multi-enzymes
Hydrogel based multi-enzymatic system for biosynthesis
Cofactor synthesis and regeneration for cell-free enzymatic cascades
The evolution with cell-free system
Cell-free synthetic biology at the interface between biology can chemistry
Rapid and finely-tuned production for deployable sensing applications
Transcriptional regulation in cell-free systems and artificial cells
Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes
Compartmentalized cell-free expression systems for bottom-up synthetic biology
Exploring the arcane cell-free as a tool to study prokaryotic and eukaryotic systems.
