001482553 000__ 04641cam\\22005297i\4500 001482553 001__ 1482553 001482553 003__ OCoLC 001482553 005__ 20231128003341.0 001482553 006__ m\\\\\o\\d\\\\\\\\ 001482553 007__ cr\un\nnnunnun 001482553 008__ 231021s2023\\\\sz\\\\\\o\\\\\000\0\eng\d 001482553 020__ $$a9783031430251 001482553 020__ $$a3031430255 001482553 020__ $$z9783031430244 001482553 0247_ $$a10.1007/978-3-031-43025-1$$2doi 001482553 035__ $$aSP(OCoLC)1405405511 001482553 040__ $$aOCLKB$$beng$$epn$$cOCLKB$$dGW5XE$$dEBLCP$$dYDX$$dOCLCO$$dOCLCF 001482553 049__ $$aISEA 001482553 050_4 $$aR856$$bC45 2023 001482553 08204 $$a610.28$$223/eng/20231024 001482553 24500 $$aCell-free production :$$bsystem development /$$cYuan Lu, Michael C. Jewett, editors. 001482553 264_1 $$aCham, Switzerland :$$bSpringer,$$c[2023] 001482553 300__ $$a1 online resource (ix, 161 pages) 001482553 336__ $$atext$$btxt$$2rdacontent 001482553 337__ $$acomputer$$bc$$2rdamedia 001482553 338__ $$aonline resource$$bcr$$2rdacarrier 001482553 4901_ $$aAdvances in biochemical engineering/biotechnology ;$$v186 001482553 5050_ $$aCell-free biomanufacturing by purified cascade multi-enzymes -- Hydrogel based multi-enzymatic system for biosynthesis -- Cofactor synthesis and regeneration for cell-free enzymatic cascades -- The evolution with cell-free system -- Cell-free synthetic biology at the interface between biology can chemistry -- Rapid and finely-tuned production for deployable sensing applications -- Transcriptional regulation in cell-free systems and artificial cells -- Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes -- Compartmentalized cell-free expression systems for bottom-up synthetic biology -- Exploring the arcane cell-free as a tool to study prokaryotic and eukaryotic systems. 001482553 506__ $$aAccess limited to authorized users. 001482553 520__ $$aThis book reviews the development of cell-free production platforms and offers an authoritative perspective of the latest advances and methodologies in cell-free production systems. Readers will discover the biomanufacturing potential of in vitro biotransformation (ivBT) employing purified cascade multi-enzymes, the development of hydrogel-based multi-enzymatic systems for biosynthesis, and novel insights into the optimization of biocatalytic processes. Additionally, the book explores the cell-free production and regeneration of cofactors, shedding light on strategies to enhance the efficiency and sustainability of cellular processes. In this book, particular attention is given to the progress of cell-free in vitro evolution techniques for optimizing enzyme performance, and the book also presents the integration of rapid and finely-tuned expression systems for deployable sensing applications, revolutionizing the field of biosensing. The synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes are explored, providing a deep understanding of cellular function at the molecular level. Lastly, the book covers compartmentalized cell-free expression systems for building synthetic cells, showcasing the potential for constructing artificial cellular systems with unique functionalities. Given its breadth, this book appeals to academics, researchers, and professionals interested in the forefront of biotechnology, and together with the companion volume Cell-free Macromolecular Synthesis, both books highlight the research progresses on the basic and applied research of cell-free production systems in the last few years, being invaluable resources in the field. Chapter Cell-free synthesis and electrophysiological analysis of multipass voltage-gated ion channels tethered in microsomal membranes is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. 001482553 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed October 24, 2023). 001482553 650_6 $$aGénie biomédical. 001482553 650_6 $$aGénie biochimique. 001482553 650_0 $$aBiomedical engineering.$$vCongresses$$0(DLC)sh2007101999 001482553 650_0 $$aBiochemical engineering. 001482553 655_0 $$aElectronic books. 001482553 7001_ $$aLu, Yuan,$$eeditor. 001482553 7001_ $$aJewett, Michael C.,$$eeditor. 001482553 830_0 $$aAdvances in biochemical engineering/biotechnology ;$$v186. 001482553 852__ $$bebk 001482553 85640 $$3Springer Nature$$uhttps://univsouthin.idm.oclc.org/login?url=https://link.springer.com/10.1007/978-3-031-43025-1$$zOnline Access$$91397441.1 001482553 909CO $$ooai:library.usi.edu:1482553$$pGLOBAL_SET 001482553 980__ $$aBIB 001482553 980__ $$aEBOOK 001482553 982__ $$aEbook 001482553 983__ $$aOnline 001482553 994__ $$a92$$bISE