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Intro
Contents
Part I: General Topics
Geothermal Habitats and Adaptations of Thermophilic Microbes
1 Introduction: Brief History of Scientific Exploration of the Upper Thermal Boundary of Life
2 Underlying Geology and Global Distribution of Geothermal Areas
2.1 High- and Low-Temperature Geothermal Areas
2.2 Surface Features in Terrestrial Geothermal Areas
3 Other High-Temperature Environments, Natural and Anthropogenic
4 Ecology in Geothermal Habitats
References
Diversity of Thermophilic Prokaryotes
1 Introduction

2 Deinococcota (Previously Deinococcus-Thermus)
2.1 Thermales
3 Nitrospirota (Previously Nitrospirae)
3.1 Nitrospirales
4 Chloroflexota (Previously Chloroflexi)
4.1 Anaerolineae
4.2 Chloroflexia
4.3 Ktedonobacteria
4.4 Other Chloroflexota Classes
5 Pseudomonadota
5.1 Acidithiobacillia
5.2 Alphaproteobacteria
5.3 Betaproteobacteria
5.4 Gammaproteobacteria
5.5 Deltaproteobacteria
6 Campylobacterota
6.1 Nautiliales
7 Firmicutes
7.1 Clostridia
7.2 Thermoanaerobacterium
7.3 Thermoanaerobacter and Caldanaerobacter
7.4 Caldicellulosiruptor

8 Thermotogae
8.1 Thermotogales
8.2 Kosmotogales
8.3 Mesoaciditogales
8.4 Petrotogales
9 Thermodesulfobacteria
10 Aquificota
10.1 Aquificae
11 Deferribacterota
11.1 Deferribacteres
12 Chlorobi
12.1 Chlorobia
12.2 Ignavibacteria
13 Thaumarchaeota
14 Archaeoglobi
15 Euryarcheota
15.1 Thermoplasmatales
15.2 Thermococcales
15.3 Methanopyrus
16 Crenarchaeota
16.1 Thermoprotei
17 Conclusions
References
Molecular Basis for Thermostability
1 Introduction
2 Adaptations to the Cellular Membrane
3 Adaptations to Nucleic Acids

4 Adaptations to Proteins
5 Future Directions
6 Conclusions
References
Cultivation Techniques and Molecular Methods of Identification of Thermophilic, Anaerobic Bacteria
1 Introduction
2 The Trouble with Oxygen
3 Cultivation of Anaerobic Bacteria
3.1 The Importance of Redox Potential
3.2 Preparation of Media for Strictly Anaerobic Bacteria
3.3 Sterilization of Media and Cultivation Vessels
3.4 Cultivation of Aerotolerant Anaerobic Bacteria
3.5 Cultivation of Strictly Anaerobic Bacteria
4 Culture-Independent Techniques for Identifying Anaerobic Bacteria

4.1 Partial Analysis of Thermophilic, Anaerobic Bacteria by Molecular Methods
4.2 Whole Community Analysis of Thermophilic, Anaerobic Bacteria by Molecular Methods
5 Conclusions
References
Part II: Biochemistry and Physiology of Thermophiles
Physiology of Chemoheterotrophic Thermoanaerobes
1 Introduction
2 Central Carbon Pathways
2.1 Catabolite Repression and Transporters
2.2 Hexose Metabolism
2.3 Pentose Metabolism
2.4 Energy Currencies
3 Cellulolytic Fermenting Thermophiles
4 Protein and Amino Acid-Degrading Thermophiles
4.1 Proteolytic Thermophiles

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