Tools and techniques of plant molecular farming / Chittaranjan Kole, Anurag Chaurasia, Kathleen L. Hefferon, Jogeswar Panigrahi, editors.
Kole, Chittaranjan.; Chaurasia, Anurag.; Hefferon, Kathleen L.; Panigrahi, Jogeswar.
2023
SB123.57
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Title Tools and techniques of plant molecular farming / Chittaranjan Kole, Anurag Chaurasia, Kathleen L. Hefferon, Jogeswar Panigrahi, editors.
ISBN 9789819948598 (electronic bk.)
9819948592 (electronic bk.)
9819948584
9789819948581
DOI https://doi.org/10.1007/978-981-99-4859-8
Publication Details Singapore : Springer, 2023.
Language English
Description 1 online resource (420 p.).
Item Number 10.1007/978-981-99-4859-8 doi
Call Number SB123.57
Dewey Decimal Classification 631.5233
Summary This edited book is an in-depth compilation of recent tools and techniques, concepts and strategies used globally in plant molecular farming (PMF) for the cost-effective bulk production of recombinant proteins, secondary metabolites, and other biomolecules. The book presents an overview of success stories of PMF applications from developing countries to address poverty, achieve zero hunger, good health and well-being, thus achieving the UN SDGs 1, 2, and 3. The book deep dives into recent extraction and downstream processing methodologies, its co-existence with conventional agriculture, global governance and finally opportunities, challenges, and future perspectives in plant molecular farming. It focuses on plastid/chloroplast transformation (transplastomics) and its application in plant molecular farming. The books highlight recent advances in genome editing, synthetic biology, glycosylation and glyco-engineering for improved plant molecular farming by marker-free and tissue-specific systems via cisgenic and transgenic crops. In depth discussions on biosafety issues and bio-containment strategies have also been included. The book has 15 chapters authored by globally leading experts on the subject, presenting opportunities & challenges for bio-industrial researchers and entrepreneurs. It is useful to researchers, industrialists, entrepreneurs, policy planners, academician, and students across the disciplines.
Note Chapter 4: Recent Genome Editing Tool-Assisted Plant Molecular Farming
Bibliography, etc. Note References -- Chapter 3: Improving Plant Molecular Farming via Genome Editing -- 3.1 Structure and Mechanism of Genome Editing Tools -- 3.2 Using Genome Editing in Plants -- 3.2.1 Using Meganucleases in Plant -- 3.2.2 Using ZFNs in Plant -- 3.2.3 Using TALENs in Plant -- 3.2.4 Using CRISPR/CAS Systems in Plant -- 3.2.4.1 CRISPR/Cas9 System -- 3.2.4.1.1 Using Cas9 as a Nuclease -- 3.2.4.1.2 Using Cas9 as a Base Editor -- 3.2.4.1.3 Using Cas9 as Prime Editors -- 3.2.4.1.4 Using Cas9 as a DNA-Binding Protein -- 3.2.4.1.5 Using Cas9 for Mediated Epigenome Editing
Access Note Access limited to authorized users.
Source of Description Online resource; title from PDF title page (SpringerLink, viewed December 11, 2023).
Added Author Kole, Chittaranjan.
Chaurasia, Anurag.
Hefferon, Kathleen L.
Panigrahi, Jogeswar.
Series Concepts and strategies in plant sciences.
Available in Other Form Tools and Techniques of Plant Molecular Farming
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Intro
Foreword
Preface
Contents
Editors and Contributors
About the Editors
Contributors
Chapter 1: Plant Molecular Farming: Concept and Strategies
1.1 Introduction
1.2 Concept
1.2.1 Plant as Expression System for Recombinant Protein Production
1.2.2 Plant as a Bioreactor for Production of Recombinant Proteins and Secondary Metabolites
1.2.2.1 Tobacco
1.2.2.2 Rice
1.2.2.3 Maize/Corn
1.2.3 Types of Recombinant Proteins Produced by PMF
1.2.3.1 Edible Vaccines
1.2.3.2 Industrial Proteins
1.2.3.3 Therapeutic Proteins

1.3 Transformation Strategies
1.3.1 Nuclear Transformation
1.3.2 Plastid Transformation
1.3.3 Agroinfiltration
1.3.4 Viral Infection
1.3.5 Magnifection System
1.4 Advantages of PMF
1.5 Challenges of PMF
1.6 Concluding Remarks
References
Chapter 2: Plant Molecular Pharming: Opportunities, Challenges, and Future Perspectives
2.1 Introduction
2.2 Opportunities of Plant Molecular Pharming
2.3 Challenges and Solutions Faced by Plant Expression Systems
2.4 Recent Examples of Recombinant Proteins Produced in Plants
2.5 Future Perspectives
2.6 Conclusions

3.2.4.1.6 Type V CRISPR/Cas12 (Cpf1) System
3.2.4.1.7 Type VI CRISPR/Cas13 Systems
3.3 Strategies to Achieve Plants via Genome Editing for Plant Molecular Farming Applications
3.3.1 DNA-Free Genome Editing/Cas9 Protein RNP Complexes In Vitro
3.3.1.1 Protoplast Transformation
3.3.1.2 Particle Bombardment
3.3.1.3 Zygotes as Delivery Targets
3.3.1.4 Nanoparticles for Cargo Delivery
3.3.2 Targeted Gene Integration Platforms
3.3.3 Improving the Yields of Recombinant Protein
3.3.3.1 Codon Preferences and tRNA Pools
3.3.3.2 Suppression of Gene Silencing

3.3.3.3 Stress Resilience and Modified Degradation Pathways
3.3.3.4 Modulation of Chaperone Expression
3.3.3.5 Modulating Endogenous Protease Activity
3.3.3.6 Modulation of Endogenous Oxidase Activity
3.3.3.7 Advanced Genome Editing of Plastid Genomes
3.3.4 Modifying Posttranslational Modifications and Product Quality
3.3.4.1 Specific N-Linked and O-Linked Glycosylation Profiles
3.3.4.2 Modifying to Avoid Toxic Metabolites or Other Disadvantageous Molecules
3.3.5 Modification of Plant Habits to Increase Space-Time Yield
3.4 Conclusions
References

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