Intro
Preface
Contents
Characterization Methods for Microbial Communities Present in Contaminated Soils
1 Introduction
2 Types of Pollutants
2.1 Inorganic Pollutants
2.2 Organic Pollutants
2.3 Biological Pollutants
2.4 Heavy Metals
2.5 Petroleum Hydrocarbons
3 Culture-Dependent Methods of Community Analysis
3.1 Dilution Plating and Culturing Methods
3.2 Community-Level Physiological Profiles
3.3 Culture-Independent Methods of Community Analysis
3.4 Phospholipid Fatty Acid Analysis
3.5 Nucleic Acid Techniques
3.6 Phylogenetic Analysis

3.7 Fluorescent in Situ Hybridization (FISH)
4 Molecular Techniques for Microbiome Analysis
4.1 Library-Based Methods
4.2 Amplification and Digestion-Based Methods
4.3 Electrophoresis-Based Methods
4.4 Sequencing-Based Methods
4.5 Fluorescence-Based Methods
4.6 Non-nucleic Acid-Based Approaches
References
Antibiotic Resistance Genes as Contaminants in Industrial Wastewater Treatment
1 Introduction
2 Antibiotics
2.1 What Is Antibiotic Resistance?
2.2 Types of Antibiotic Resistance
2.3 Working of Antibiotics

2.4 Antibiotic Resistance Development in Wastewater Bacteria
2.5 Mechanism
2.6 Factors Affecting the Spread of Antibiotic Resistance Genes in Industrial Wastewater
2.7 Are Wastewater Treatment Plants a Solution or a Culprit?
3 How Are Antibiotic Resistance Genes Quantified?
3.1 Quantitative Polymerase Chain Reaction (qPCR)
3.2 Fluorescence in Situ Hybridization (FISH) Technique
3.3 Culture Based
3.4 Metagenomics
4 Standardizing the Levels of ARGs into Risk Groups
4.1 Drinking Water
4.2 Air and Dust
4.3 Soil
4.4 Food

5 Steps to Address Antibiotic Resistance Genes Contamination Possible Measures to Be Targeted
6 Who Needs to Take Action?
7 Conclusion
References
Bacteriophages: A Strategy to Combat Antibiotic Resistance in Wastewater Treatment Plants
1 Introduction
2 Occurrence of AMR in WWTPs
3 Antibiotics Leading to Resistance Among Bacteria
4 Dissemination of Antibiotic Resistance Among Bacteria
5 Sources of Antibiotic Resistant Bacteria (ARB)
6 Bacteriophage
7 Bacteriophage Life Cycle
8 Studies of Phages Against ARBs
9 Conclusion
References

The Emergence of Wastewater Treatment Plant as a Leading Source for Dissemination of Antibiotic-Resistant Gene
1 Introduction
2 Antibiotic-Resistant Genes and Its Dissemination in Wastewater Treatment Plant
3 Different ARGs Available in Wastewater Treatment Plants
3.1 Beta-Lactam Resistant Genes
3.2 Macrolide Resistance Genes
3.3 Quinolone Resistance Genes
3.4 Sulfonamide and Trimethoprim Resistance Genes
4 Sources of Antibiotic-Resistant Genes
5 Role of Wastewater Treatment Plant in the Dissemination of ARGs
6 Different Strategies for Treating the ARGs by WWTPs