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Pt.1. Basic principles and methods
What is PCR?
Instrumentation, reagents and consumables
Amplifying the correct product
Pt.2. Techniques and applications
Cloning and modification of PCR products
Isolation and construction of DNA clones
PCR mutagenesis
Sequencing PCR products
New sequence determination and genome mapping
Figerprinting
Characterizing unknown mutations
Analysis of known mutations
Detecting pathogens
Quantitative PCR
Appendices
Index.

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