000695828 000__ 02845cam\a2200469Ki\4500 000695828 001__ 695828 000695828 005__ 20230306135459.0 000695828 006__ m\\\\\o\\d\\\\\\\\ 000695828 007__ cr\cnu---unuuu 000695828 008__ 131120s2014\\\\sz\a\\\\om\\\\000\0\eng\d 000695828 020__ $$a9783319021591 $$qelectronic book 000695828 020__ $$a3319021591 $$qelectronic book 000695828 020__ $$z9783319021584 000695828 0247_ $$a10.1007/978-3-319-02159-1$$2doi 000695828 035__ $$aSP(OCoLC)ocn863379270 000695828 035__ $$aSP(OCoLC)863379270 000695828 040__ $$aGW5XE$$beng$$erda$$epn$$cGW5XE$$dN$T$$dYDXCP$$dCOO 000695828 049__ $$aISEA 000695828 050_4 $$aQH212.E4 000695828 08204 $$a502.825$$223 000695828 1001_ $$aSharp, Thomas H.,$$eauthor. 000695828 24510 $$aBiomolecular imaging at high spatial and temporal resolution in vitro and in vivo$$h[electronic resource] /$$cby Thomas H. Sharp. 000695828 264_1 $$aCham :$$bSpringer,$$c2014. 000695828 300__ $$a1 online resource. 000695828 336__ $$atext$$btxt$$2rdacontent 000695828 337__ $$acomputer$$bc$$2rdamedia 000695828 338__ $$aonline resource$$bcr$$2rdacarrier 000695828 4901_ $$aSpringer theses 000695828 502__ $$bPh.D.$$cUniversity of Bristol$$d[2012?] 000695828 5050_ $$aBiomolecules & Microscopy -- Materials and Methods -- Experimental Methods -- Imaging Methods -- Experimental Results -- Towards an Atomistic Structure of a De Novo Designed Peptide Fibre -- Developing Novel Probes for Correlative Light Electron Microscopy. 000695828 506__ $$aAccess limited to authorized users. 000695828 520__ $$aAs part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his PhD, Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis. 000695828 588__ $$aDescription based on print version record. 000695828 650_0 $$aElectron microscopy. 000695828 650_0 $$aCryobiochemistry. 000695828 650_0 $$aPeptides$$xStructure. 000695828 77608 $$iPrint version:$$aSharp, Thomas H., author.$$tBiomolecular imaging at high spatial and temporal resolution in vitro and in vivo$$z9783319021584$$w(OCoLC)861648930 000695828 830_0 $$aSpringer theses. 000695828 85280 $$bebk$$hSpringerLink 000695828 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://dx.doi.org/10.1007/978-3-319-02159-1$$zOnline Access 000695828 909CO $$ooai:library.usi.edu:695828$$pGLOBAL_SET 000695828 980__ $$aEBOOK 000695828 980__ $$aBIB 000695828 982__ $$aEbook 000695828 983__ $$aOnline 000695828 994__ $$a92$$bISE