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000725080 020__ $$a9783319123974$$qelectronic book
000725080 020__ $$a3319123971$$qelectronic book
000725080 020__ $$z9783319123967
000725080 0247_ $$a10.1007/978-3-319-12397-4$$2doi
000725080 035__ $$aSP(OCoLC)ocn899495715
000725080 035__ $$aSP(OCoLC)899495715
000725080 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dGW5XE$$dE7B$$dYDXCP$$dUPM$$dOCLCF$$dDKU$$dN$T$$dIDEBK$$dCOO$$dEBLCP$$dVLB
000725080 049__ $$aISEA
000725080 050_4 $$aTP248.E5$$bR43 2015eb
000725080 08204 $$a572/.7$$223
000725080 24500 $$aRecombinant enzymes-- from basic science to commercialization$$h[electronic resource] /$$cAzura Amid, editor.
000725080 264_1 $$aCham :$$bSpringer,$$c2015.
000725080 300__ $$a1 online resource (x, 188 pages) :$$billustrations
000725080 300__ $$a1 online resource.
000725080 336__ $$atext$$btxt$$2rdacontent
000725080 337__ $$acomputer$$bc$$2rdamedia
000725080 338__ $$aonline resource$$bcr$$2rdacarrier
000725080 500__ $$aIncludes index.
000725080 5050_ $$aIntroduction to Recombinant Enzyme Pre-commercialization -- Recombinant Enzyme: Cloning and Expression -- Common Laboratory Procedure -- Characterization of Recombinant Enzymes -- Purification of Recombinant Protein for Industrial Use -- Recombinant Enzyme Fermentation -- Scaling-up Recombinant Enzyme Fermentation -- Downstream Processing of Recombinant Enzymes for Commercialization -- Economic and Environmental Evaluation of Recombinant Enzyme Production -- Case Study: Recombinant Bromelain Selection -- Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes -- Case Study: Recombinant Bromelain Downstream Processing.
000725080 506__ $$aAccess limited to authorized users.
000725080 520__ $$aThis edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product.℗ℓ Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host.℗ℓ A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production.℗ℓ This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.℗ℓ
000725080 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed January 26, 2015).
000725080 650_0 $$aEnzymes.
000725080 650_0 $$aRecombinant DNA.
000725080 7001_ $$aAmid, Azura,$$eeditor.
000725080 77608 $$iPrint version:$$z9783319123967
000725080 852__ $$bebk
000725080 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-319-12397-4$$zOnline Access$$91397441.1
000725080 909CO $$ooai:library.usi.edu:725080$$pGLOBAL_SET
000725080 980__ $$aEBOOK
000725080 980__ $$aBIB
000725080 982__ $$aEbook
000725080 983__ $$aOnline
000725080 994__ $$a92$$bISE