000725429 000__ 05356cam\a2200493Ii\4500 000725429 001__ 725429 000725429 005__ 20230306140638.0 000725429 006__ m\\\\\o\\d\\\\\\\\ 000725429 007__ cr\cn\nnnunnun 000725429 008__ 150130s2015\\\\nyu\\\\\ob\\\\000\0\eng\d 000725429 020__ $$a9781493923465$$qelectronic book 000725429 020__ $$a1493923463$$qelectronic book 000725429 020__ $$z9781493923458 000725429 0247_ $$a10.1007/978-1-4939-2346-5$$2doi 000725429 035__ $$aSP(OCoLC)ocn901263373 000725429 035__ $$aSP(OCoLC)901263373 000725429 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dN$T$$dGW5XE$$dYDXCP$$dCOO$$dIDEBK$$dE7B$$dEBLCP$$dCDX$$dVLB 000725429 049__ $$aISEA 000725429 050_4 $$aQR82.E6$$bR58 2015eb 000725429 08204 $$a579.3/42$$223 000725429 1001_ $$aRivas, Lucia,$$eauthor. 000725429 24510 $$aDetection and typing strategies for pathogenic escherichia coli$$h[electronic resource] /$$cLucia Rivas, Glen E. Mellor, Kari Gobius, Narelle Fegan. 000725429 264_1 $$aNew York :$$bSpringer,$$c[2015] 000725429 264_4 $$c©2015 000725429 300__ $$a1 online resource 000725429 336__ $$atext$$btxt$$2rdacontent 000725429 337__ $$acomputer$$bc$$2rdamedia 000725429 338__ $$aonline resource$$bcr$$2rdacarrier 000725429 4901_ $$aSpringerBriefs in food, health, and nutrition 000725429 504__ $$aIncludes bibliographical references. 000725429 5050_ $$aChapter 1 Introduction to Pathogenic Escherichia coli -- 1.1 Pathotypes of Escherichia coli causing diarrhoeal diseases -- 1.2 Escherichia coli -- 1.3 Pathogenic types of E. coli -- 1.4 Shiga toxin-producing and Enterohemorrhagic E. coli -- 1.4.1 Virulence determinants of Shiga-toxin producing E. coli -- 1.4.2 Shiga-toxin producing E. coli-mediated disease -- 1.4.3 Epidemiology of Shiga toxin-producing E. coli -- 1.4.4 Ecology of Shiga-toxin producing E. coli -- 1.4.5 Transmission of Shiga-toxin producing E. coli -- 1.4.6 Shiga-toxin producing E. coli occurrence in foods -- 1.5 Enterotoxigenic E. coli -- 1.6 Enteropathogenic E. coli -- 1.7 Enteroinvasive E. coli -- 1.8 Enteroaggregative and Diffusely Adherent E. coli..-1.9 References -- Chapter 2 Isolation and detection of pathogenic Escherichia coli in foods -- 2.1 Introduction -- 2.2 General method of isolation for E. coli -- 2.3 Shiga toxin-producing E. coli -- 2.3.1 Culture and isolation of Shiga toxin-producing E. coli -- 2.3.2 Molecular detection of Shiga toxin-producing E. coli -- 2.4 Enumeration of Shiga toxin-producing E.coli -- 2.5 Immunological detection methods for pathogenic E. coli -- 2.6 Cell culture assays used for pathogenic E. coli -- 2.6.1 Cell cytotoxicity assay for Shiga toxin-producing E. coli -- 2.6.2 Cell adherence assays -- 2.7 Enteroinvasive E. coli -- 2.8 Enterotoxigenic E. coli -- 2.9 Enteropathogenic E. coli -- 2.10 Enteroaggregative E. coli and Diffusely Adherent E. coli -- 2.11 References -- Chapter 3 Typing and Subtyping methods for pathogenic Escherichia coli -- 3.1 Introduction -- 3.2 Biochemical profiling -- 3.3 Serotyping -- 3.4 Phage typing -- 3.5 Multilocus enzyme electrophoresis -- 3.6 Multilocus sequence typing -- 3.7 Pulsed-field gel electrophoresis -- 3.8 Multiple-locus variable number tandem repeat analysis -- 3.9 Repetitive element palindromic polymerase chain reaction -- 3.10 Random amplified polymorphic DNA -- 3.11 Shiga toxin subtypes and bacteriophage insertion sites -- 3.12 Lineage specific polymorphism analysis -- 3.13 Whole genome sequencing -- 3.14 -- References -- Chapter 4 Emerging and future trends and technologies for the detection and typing of Escherichia coli -- 4.1 Future trends in foodborne illness and E. coli -- 4.2 Future trends in the detection and typing of pathogens -- 4.2.1 Microarrays -- 4.2.2 Matrix Assisted Laser-Desorption Ionization-Time-Of-Flight Mass Spectrometry -- 4.2.3 Nanotechnology -- 4.2.4 Next-generating sequencing of the whole bacterial genome -- 4.3 References. 000725429 506__ $$aAccess limited to authorized users. 000725429 520__ $$aThis Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. Pathogenic forms of E. coli can cause a variety of diarrheal diseases in hosts due to the presence of specific colonization and virulence factors, and pathogenicity-associated genes, which are generally not present in other E. coli. Six pathotypes of pathogenic E. coli are recognized (Shiga toxigenic E. coli, Enteropathogenic E. coli, Enterotoxigenic E. coli, Enteroinvasive E. coli, Enteroaggregative E. coli and Diffusely Adherent E. coli) and certain strains among these groups are major public health concerns due to the severity of disease that they can cause. Methods to detect and isolate these pathogens from a variety of sources are constantly evolving. In addition, the accumulation of knowledge on these pathogens allows for improved intervention strategies. 000725429 650_0 $$aEscherichia coli. 000725429 650_0 $$aEscherichia coli$$xMicrobiology. 000725429 7001_ $$aMellor, Glen E.$$eauthor. 000725429 7001_ $$aGobius, Kari,$$eauthor. 000725429 7001_ $$aFegan, Narelle,$$eauthor. 000725429 77608 $$iPrint version:$$z9781493923458 000725429 830_0 $$aSpringerBriefs in food. health. and nutrition. 000725429 852__ $$bebk 000725429 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-1-4939-2346-5$$zOnline Access$$91397441.1 000725429 909CO $$ooai:library.usi.edu:725429$$pGLOBAL_SET 000725429 980__ $$aEBOOK 000725429 980__ $$aBIB 000725429 982__ $$aEbook 000725429 983__ $$aOnline 000725429 994__ $$a92$$bISE