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000726068 019__ $$a908097971
000726068 020__ $$a9783319162539$$qelectronic book
000726068 020__ $$a3319162535$$qelectronic book
000726068 020__ $$z9783319162522
000726068 0247_ $$a10.1007/978-3-319-16253-9$$2doi
000726068 035__ $$aSP(OCoLC)ocn904979114
000726068 035__ $$aSP(OCoLC)904979114$$z(OCoLC)908097971
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000726068 049__ $$aISEA
000726068 050_4 $$aQP623.8.P75
000726068 08204 $$a572.8/8$$223
000726068 1001_ $$aMunschauer, Mathias,$$eauthor.
000726068 24510 $$aHigh-resolution profiling of protein-RNA interactions$$h[electronic resource] /$$cMathias Munschauer.
000726068 264_1 $$aCham :$$bSpringer,$$c[2015]
000726068 300__ $$a1 online resource :$$bcolor illustrations.
000726068 336__ $$atext$$btxt$$2rdacontent
000726068 337__ $$acomputer$$bc$$2rdamedia
000726068 338__ $$aonline resource$$bcr$$2rdacarrier
000726068 4901_ $$aSpringer theses,$$x2190-5061
000726068 500__ $$a"Doctoral thesis accepted by Freie Universität Berlin, Germany."
000726068 504__ $$aIncludes bibliographical references.
000726068 5050_ $$aIntroduction -- Mapping Regulatory Interactions of the Rna-Binding Protein Lin28b -- Exploring The Sequence Space Contacted by the Ensemble of Rna-Binding Proteins -- Revealing Cell-Type Specific Differences in Protein Occupancy on Rna -- Discussion.
000726068 506__ $$aAccess limited to authorized users.
000726068 520__ $$aThe work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ?protein occupancy profiling? technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3? UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.
000726068 588__ $$aOnline resource; title from PDF title page (viewed March 18, 2015).
000726068 650_0 $$aRNA-protein interactions.
000726068 650_0 $$aRNA polymerases.
000726068 77608 $$iPrint version:$$z9783319162522
000726068 830_0 $$aSpringer theses.
000726068 852__ $$bebk
000726068 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-319-16253-9$$zOnline Access$$91397441.1
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