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000752640 019__ $$a930836932$$a931593424$$a932170602$$a932333826
000752640 020__ $$a9784431557234$$q(electronic book)
000752640 020__ $$a4431557237$$q(electronic book)
000752640 020__ $$z9784431557227
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000752640 0247_ $$a10.1007/978-4-431-55723-4$$2doi
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000752640 08204 $$a616.0754$$223
000752640 24500 $$aIn vivo cryotechnique in biomedical research and application for bioimaging of living animal organs$$h[electronic resource] /$$cShinichi Ohno, Nobuhiko Ohno, Nobuo Terada, editors.
000752640 264_1 $$aTokyo :$$bSpringer,$$c[2016]
000752640 264_4 $$c©2016
000752640 300__ $$a1 online resource.
000752640 336__ $$atext$$btxt$$2rdacontent
000752640 337__ $$acomputer$$bc$$2rdamedia
000752640 338__ $$aonline resource$$bcr$$2rdacarrier
000752640 347__ $$atext file$$bPDF$$2rda
000752640 504__ $$aIncludes bibliographical references and index.
000752640 5050_ $$aPreface; Contents; Part I: Overview; 1: Introduction; References; 2: Biomedical Significance and Development of "IVCT"; 2.1 Inevitable Problems with Conventional Preparation Techniques; 2.2 Quick-Freezing (QF) Method for Resected Fresh Tissues; 2.3 Development of In Vivo Cryotechnique (IVCT); References; 3: How to Perform "IVCT"; 3.1 Introduction; 3.2 Practical Procedures of In Vivo Cryoapparatus; 3.3 IVCT for Light Microscopy; 3.4 How to Prepare the Mixed Isopentane-Propane Cryogen; References; 4: Technical Merits with "IVCT"; 4.1 Tissue Depth of Well-Frozen Areas.
000752640 5058_ $$a4.2 Plunging or Slamming Cryotechniques4.3 Thermal Conductance; References; Part II: Application of 'IVCT' to Various Mouse Organs; 5: Histochemical Analyses of Living Mouse Liver Under Different Hemodynamic Conditions; 5.1 Introduction; 5.2 Morphological Features of Living Mouse Liver; 5.3 Distribution of Serum Proteins and Glycogen; 5.4 Disrupted Cell Membrane Integrity; 5.5 Concluding Remarks; References; 6: Three-Dimensional Reconstruction of Liver Tissues with Confocal Laser Scanning Microscopy; 6.1 Introduction.
000752640 5058_ $$6880-01$$a9: Detection of MAPK Signal Transduction Proteins in an Ischemia/Reperfusion Model of Small Intestines9.1 Introduction; 9.2 Histology and Immunolocalization of ERK1/2 and pERK1/2 in Normal Mouse Small Intestine by Various Preparative Methods; 9.3 ERK1/2 and pERK1/2 in 20 min Ischemia Group with IVCT-FS and IM-DH; 9.4 ERK1/2 and pERK1/2 in 20 min Ischemia with 60 min Reperfusion Group Prepared by IVCT-FS; 9.5 Concluding Remarks; References; 10: Histological Study and LYVE-1 Immunolocalization of Mesenteric Lymph Nodes; 10.1 Introduction.
000752640 506__ $$aAccess limited to authorized users.
000752640 520__ $$aThis book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.
000752640 588__ $$aOnline resource; title from PDF title page (viewed December 16, 2015).
000752640 650_0 $$aImaging systems in biology.
000752640 650_0 $$aImaging systems in medicine.
000752640 650_0 $$aMedicine$$xResearch.
000752640 7001_ $$aOhno, Shinichi,$$eeditor.
000752640 7001_ $$aOhno, Nobuhiko,$$eauthor.
000752640 7001_ $$aTerada, Nobuo,$$eauthor.
000752640 77608 $$iPrint version:$$z9784431557227
000752640 852__ $$bebk
000752640 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-4-431-55723-4$$zOnline Access$$91397441.1
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000752640 980__ $$aEBOOK
000752640 980__ $$aBIB
000752640 982__ $$aEbook
000752640 983__ $$aOnline
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