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Preface; Contents; Chapter 1: Studying Protein Misfolding and Aggregation by Fluorescence Spectroscopy; 1.1 A Brief Introduction to Protein Misfolding and Aggregation; 1.2 Experimental Techniques to Study Protein Aggregation; 1.3 Fluorescence Spectroscopy and Protein Aggregation; 1.4 Fluorescence Techniques to Study Protein Oligomers and Amyloids During Protein Aggregation; 1.4.1 Fluorescence Intensity, Spectrum and Lifetime; 1.4.1.1 Intrinsic Fluorophores; 1.4.1.2 Extrinsic Fluorophores; 1.4.2 Fluorescence Polarization (Anisotropy); 1.4.3 Fluorescence Quenching.
1.4.4 Fluorescence Resonance Energy Transfer (FRET)1.4.5 Single-Molecule Fluorescence Studies; 1.4.6 Fluorescence Correlation Spectroscopy (FCS); 1.5 Case Studies from our Laboratory at IISER Mohali; 1.5.1 Lysozyme Aggregation; 1.5.2 Serum Albumin Aggregation; 1.5.3 Conformational Property of kappa-Casein
A Model Intrinsically Disordered Protein; 1.6 Conclusions and Future Directions; References; Chapter 2: Time-Dependent Spectral Shifts in Tryptophan Fluorescence: Bridging Experiments with Molecular Dynamics Simulations; 2.1 Introduction.
2.2 Relationship Between Spectral Shifts and Electronic Energy Levels2.3 Effect of the Electric Field on the Electronic Energy Levels; 2.4 Nonlinear Stark Effect; 2.5 Hybrid QM-MD Versus Classical MD; 2.6 Direct-Response Versus Linear-Response Method; 2.7 Separation of Contributions from Different Motions; 2.8 Dielectric Relaxation of Bulk Solvent; 2.9 Relaxation of Water Molecules Near Protein Surface and Inside Protein; 2.10 Separation of Relaxation Modes by Their Time Scales; 2.11 Conclusions; References; Chapter 3: Directional Fluorescence Based on Surface Plasmon-Coupling.
3.1 Introduction3.2 Properties of SPCE; 3.2.1 Directional Emission; 3.2.2 Background Suppression; 3.2.3 P-Polarized Emission; 3.2.4 Distance-Determined Coupling; 3.3 The Strategies for Improving SPCE; 3.3.1 Enlarging the Optical Window; 3.3.2 Improving the Applicability and Sensitivity; 3.3.3 Obtaining Controllable Coupling with Spatial Resolution; 3.4 SPCE Optical Imaging; 3.4.1 SPCE Imaging System Based on Microscope Technique; 3.4.2 Point Spread Function of SPCE Microscope; 3.4.3 Present Researches Utilizing Optical Imaging; 3.5 The Applications of SPCE in Analysis; 3.5.1 DNA Sensing.
3.5.2 Aptamers-Based Protein Sensing3.5.3 Immunological Detection; 3.5.4 Other Fluorescence Analysis; 3.6 Conclusions; References; Chapter 4: Fluorescence Analysis of Thermoresponsive Polymers; 4.1 Introduction; 4.2 Stimuli-Responsive Polymers; 4.2.1 Thermoresponsive Polymers; 4.2.2 Poly(N-IsoPropylAcrylamide), PNIPAm; 4.3 Polymer Characterisation by Fluorescence; 4.4 Thermoresponsive Polymer Characterization; 4.4.1 Water Sorption; 4.4.2 Physicochemical Parameters by Solvatochromism; 4.4.3 Physicochemical Parameters by Fluorescence.
1.4.4 Fluorescence Resonance Energy Transfer (FRET)1.4.5 Single-Molecule Fluorescence Studies; 1.4.6 Fluorescence Correlation Spectroscopy (FCS); 1.5 Case Studies from our Laboratory at IISER Mohali; 1.5.1 Lysozyme Aggregation; 1.5.2 Serum Albumin Aggregation; 1.5.3 Conformational Property of kappa-Casein
A Model Intrinsically Disordered Protein; 1.6 Conclusions and Future Directions; References; Chapter 2: Time-Dependent Spectral Shifts in Tryptophan Fluorescence: Bridging Experiments with Molecular Dynamics Simulations; 2.1 Introduction.
2.2 Relationship Between Spectral Shifts and Electronic Energy Levels2.3 Effect of the Electric Field on the Electronic Energy Levels; 2.4 Nonlinear Stark Effect; 2.5 Hybrid QM-MD Versus Classical MD; 2.6 Direct-Response Versus Linear-Response Method; 2.7 Separation of Contributions from Different Motions; 2.8 Dielectric Relaxation of Bulk Solvent; 2.9 Relaxation of Water Molecules Near Protein Surface and Inside Protein; 2.10 Separation of Relaxation Modes by Their Time Scales; 2.11 Conclusions; References; Chapter 3: Directional Fluorescence Based on Surface Plasmon-Coupling.
3.1 Introduction3.2 Properties of SPCE; 3.2.1 Directional Emission; 3.2.2 Background Suppression; 3.2.3 P-Polarized Emission; 3.2.4 Distance-Determined Coupling; 3.3 The Strategies for Improving SPCE; 3.3.1 Enlarging the Optical Window; 3.3.2 Improving the Applicability and Sensitivity; 3.3.3 Obtaining Controllable Coupling with Spatial Resolution; 3.4 SPCE Optical Imaging; 3.4.1 SPCE Imaging System Based on Microscope Technique; 3.4.2 Point Spread Function of SPCE Microscope; 3.4.3 Present Researches Utilizing Optical Imaging; 3.5 The Applications of SPCE in Analysis; 3.5.1 DNA Sensing.
3.5.2 Aptamers-Based Protein Sensing3.5.3 Immunological Detection; 3.5.4 Other Fluorescence Analysis; 3.6 Conclusions; References; Chapter 4: Fluorescence Analysis of Thermoresponsive Polymers; 4.1 Introduction; 4.2 Stimuli-Responsive Polymers; 4.2.1 Thermoresponsive Polymers; 4.2.2 Poly(N-IsoPropylAcrylamide), PNIPAm; 4.3 Polymer Characterisation by Fluorescence; 4.4 Thermoresponsive Polymer Characterization; 4.4.1 Water Sorption; 4.4.2 Physicochemical Parameters by Solvatochromism; 4.4.3 Physicochemical Parameters by Fluorescence.