Preface; Contents; Chapter 1: Immunohistochemistry: Overview, Its Potential, and Challenges; Overview; The Potential of IHC; Challenges; Conclusion; References; Chapter 2: Tissue Microarray Technology and Its Potential Applications in Toxicology and Toxicological Immunohistochemistry; Introduction; The Emergence and Dominance of Post-genomic Technologies; The Proliferation of Microarray Technologies; Tissue Microarrays; What Are TMAs?; Why Use TMAs?; How Is TMAs Produced?; TMA Resources; CHTN TMAs; The Tissue Array Research Program; National Disease Research Interchange.

Commercially Produced TMAs Bioinformatic Approaches for TMA Data Analysis; TMAs for Toxicological Research: Opportunities and Challenges; Applications in Toxicology; Conclusions; References; Chapter 3: Importance of Fluorescent In Situ Hybridization in Rodent Tumors; Introduction; Fluorescent In Situ Hybridization: A Conceptual Approach; DNA Probes; Single-Copy Probes; Whole-Genome Analysis in a Single Experiment with FISH-Based Technologies; Multiplex-FISH and Spectral Karyotyping Analysis; Comparative Genomic Hybridization Analyses; Microarrays Based Cytogenetics.

Multicolor Banding Analysis of Centromeres and Telomeres in Tumors; Combining Approaches: An Improvement in Cytogenetic Characterization; RNA Analysis in Single Cells; Concluding Remarks; References; Chapter 4: Quantitation of Immunohistochemistry by Image Analysis Technique; Introduction; Tissue Microarrays; Tissue-Based Diagnosis and Types of Diagnoses; Basics of Image Analysis; Principles of Microscopic Image Analysis; Colour Space; Image Quality; Object Segmentation; Syntactic Structure Algorithms; Image Information and Feature Extraction; Region of Interest.

Application in IHC Perspectives; References; Chapter 5: Immunohistochemistry in the Study of Cancer Biomarkers for Oncology Drug Development; Introduction; Technical Aspects of IHC; Basic Procedure of IHC; Fixation; FFPE Tissue Section and Frozen Fresh Tissue Cryosection; Tissue Pretreatment and Antigen Retrieval; Blocking Considerations; Primary Antibody and Controls; Detection Systems; Direct Methods; Indirect Method; Combination of Detection Systems for Multiple Target Protein Detection; Chromagens; Counterstaining; Coverslip; IHC for Cancer Biomarker Studies.

Cancer Biomarker Studies on Tissue Section Slides Cancer Biomarker Study on Cytological Preparation Slides; Assay Development; General Assay Development and Validation; Assay Development of Multi-fluorophore Labeling Techniques; Tumor Vascular Measurement; Circulating Tumor Cell Assessment; IHC Score; Score for Tumor Vasculature; Quantification of Microvessels and Target Receptors; Quantification of Hypoxic Protein Expression; Quantification of Pericyte Coverage; Score for Circulating Tumor Cells; Statistical Analysis; Companion Diagnostics; Sources of IHC Errors and Troubleshooting.