000753480 000__ 04029cam\a2200481Ii\4500 000753480 001__ 753480 000753480 005__ 20230306141600.0 000753480 006__ m\\\\\o\\d\\\\\\\\ 000753480 007__ cr\cn\nnnunnun 000753480 008__ 160126s2016\\\\gw\a\\\\ob\\\\000\0\eng\d 000753480 020__ $$a9783662491188$$q(electronic book) 000753480 020__ $$a3662491184$$q(electronic book) 000753480 020__ $$z9783662491164 000753480 0247_ $$a10.1007/978-3-662-49118-8$$2doi 000753480 035__ $$aSP(OCoLC)ocn936040264 000753480 035__ $$aSP(OCoLC)936040264 000753480 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dN$T$$dYDXCP$$dGW5XE$$dIDEBK$$dEBLCP$$dAZU$$dCOO$$dOCLCF$$dOCLCQ 000753480 049__ $$aISEA 000753480 050_4 $$aQH585 000753480 08204 $$a571.6$$223 000753480 24500 $$aEssentials of single-cell analysis$$h[electronic resource] :$$bconcepts, applications and future prospects /$$cFan-Gang Tseng, Tuhin Subhra Santra, editors. 000753480 264_1 $$aBerlin :$$bSpringer,$$c[2016] 000753480 300__ $$a1 online resource :$$billustrations. 000753480 336__ $$atext$$btxt$$2rdacontent 000753480 337__ $$acomputer$$bc$$2rdamedia 000753480 338__ $$aonline resource$$bcr$$2rdacarrier 000753480 4901_ $$aSeries in bioEngineering 000753480 504__ $$aIncludes bibliographical references. 000753480 5050_ $$aSingle Cell Behavioral Assays for Heterogeneity Studies -- Systems biology in single cells -- Electroporation for single cell analysis -- Microinjection for single cell analysis -- Optical tools for single cell manipulation and analysis -- Optoelectrokinetic manipulation for cell analysis -- Continuous micro/nano-fluidic devices for single cell analysis -- Single Cell Mechanical Properties: Label-Free Biomarkers for Cell Status Evaluation -- Cytometry of single cells for biology and biomedicine -- Single cell genomics and epigenomics -- Single cell Metabolomics -- Applications of cell based drug delivery systems: use of single cell assay -- Applications of Single Cell Sequencing in Cancer -- Single Cell Characterization of Microalgal Lipid Contents with Confocal Raman Microscopy -- Single Differentiated Neurons from Pluripotent Embryonic Stem Cells: Motor Protein Modelling and Neurodegenerative Disease. 000753480 506__ $$aAccess limited to authorized users. 000753480 520__ $$aThis book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA. 000753480 588__ $$aOnline resource; title from PDF title page (viewed January 27, 2016). 000753480 650_0 $$aCytology$$xTechnique. 000753480 650_0 $$aCells$$xAnalysis. 000753480 650_0 $$aCytology$$xResearch. 000753480 7001_ $$aTseng, Fan-Gang,$$eeditor. 000753480 7001_ $$aSantra, Tuhin Subhra,$$eeditor. 000753480 77608 $$iPrint version:$$z9783662491164 000753480 830_0 $$aSeries in bioengineering. 000753480 852__ $$bebk 000753480 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-662-49118-8$$zOnline Access$$91397441.1 000753480 909CO $$ooai:library.usi.edu:753480$$pGLOBAL_SET 000753480 980__ $$aEBOOK 000753480 980__ $$aBIB 000753480 982__ $$aEbook 000753480 983__ $$aOnline 000753480 994__ $$a92$$bISE