@article{755301,
      recid = {755301},
      author = {Vega, M. Cristina,},
      title = {Advanced technologies for protein complex production and  characterization [electronic resource] /},
      pages = {1 online resource (xiii, 382 pages) :},
      note = {Includes index.},
      abstract = {This book presents advanced expression technologies for  the production of protein complexes. Since complexes lie at  the heart of modern biology, the expression, purification,  and characterization of large amounts of high-quality  protein complexes is crucial for the fields of biomedicine,  biotechnology, and structural biology. From co-expression  in E. coli, yeast, mammalian and insect cells to complex  reconstitution from individual subunits, this book offers  useful insights and guidance for successful protein  expressionists. Across several sections readers will  discover existing opportunities for the production of  protein complexes in bacterial systems (including membrane  proteins and cell-free co-expression), methylotrophic and  non-methylotrophic yeasts, protozoa (Leishmania terantolae  and Dictyostelium discoideum), baculovirus-infected insect  cells, mammalian cells, plants and algae. Complex  reconstitution from individually purified subunits or  subcomplexes is discussed as a complementary strategy. A  last section introduces briefly some of the biophysical and  structural characterization techniques for macromolecular  complexes using state-of-the-art solution scattering and  nuclear magnetic resonance. This work is a guided tour over  some of the most powerful and successful protein expression  technologies, with a focus on co-expression and  high-throughput applications. It is addressed to everyone  interested in the production and characterization of  macromolecular complexes, from university students who want  an accessible description of the major co-expression  systems to researchers in biomedicine and the life sciences  seeking for an up-to-date survey of available  technologies.},
      url = {http://library.usi.edu/record/755301},
}