000755301 000__ 05139cam\a2200445Ii\4500
000755301 001__ 755301
000755301 005__ 20230306141841.0
000755301 006__ m\\\\\o\\d\\\\\\\\
000755301 007__ cr\cn\nnnunnun
000755301 008__ 160512s2016\\\\sz\a\\\\o\\\\\001\0\eng\d
000755301 020__ $$a9783319272160$$q(electronic book)
000755301 020__ $$a3319272160$$q(electronic book)
000755301 020__ $$z9783319272146
000755301 035__ $$aSP(OCoLC)ocn949668645
000755301 035__ $$aSP(OCoLC)949668645
000755301 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dYDXCP$$dGW5XE$$dN$T$$dIDEBK$$dOCLCO$$dEBLCP$$dOCLCF$$dOCLCO$$dAZU
000755301 049__ $$aISEA
000755301 050_4 $$aTP248.65.P76
000755301 08204 $$a572/.6$$223
000755301 24500 $$aAdvanced technologies for protein complex production and characterization$$h[electronic resource] /$$cM. Cristina Vega, editor.
000755301 264_1 $$aCham :$$bSpringer,$$c2016.
000755301 300__ $$a1 online resource (xiii, 382 pages) :$$billustrations.
000755301 336__ $$atext$$btxt$$2rdacontent
000755301 337__ $$acomputer$$bc$$2rdamedia
000755301 338__ $$aonline resource$$bcr$$2rdacarrier
000755301 4901_ $$aAdvances in experimental medicine and biology,$$x0065-2598 ;$$vvolume 896
000755301 500__ $$aIncludes index.
000755301 5050_ $$aProtein complex production from the drug discovery standpoint -- Choose a suitable expression host: a survey of available protein production platforms -- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts -- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput -- Membrane protein production in E. coli for applications in drug discovery -- Cell-free Synthesis of Macromolecular Complexes -- A Bacillus megaterium system for the production of recombinant proteins and protein complexes -- Protein complex production in alternative prokaryotic hosts -- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts -- Leishmania tarentolae for the Production of Multi-Subunit Complexes -- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes -- Fundamentals of baculovirus expression and applications -- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics -- Fundamentals of expression in mammalian cells -- Assembling multi-subunit complexes using mammalian expression -- Microalgae as solar-powered protein factories -- Strategies and methodologies for the co-expression of multiple proteins in plants -- Transient expression systems in plants -- potentialities and constraints -- Complex reconstitution from individual protein modules -- Structural reconstruction of protein-protein complexes involved in intracellular signaling -- The use of small-angle scattering for the characterization of multi subunit complexes -- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. .
000755301 506__ $$aAccess limited to authorized users.
000755301 520__ $$aThis book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.
000755301 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed May 18, 2016).
000755301 650_0 $$aProtein engineering.
000755301 650_0 $$aProteins$$xBiotechnology.
000755301 650_0 $$aProtein drugs.
000755301 7001_ $$aVega, M. Cristina,$$eeditor.
000755301 830_0 $$aAdvances in experimental medicine and biology ;$$vv. 896.
000755301 852__ $$bebk
000755301 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-319-27216-0$$zOnline Access$$91397441.1
000755301 909CO $$ooai:library.usi.edu:755301$$pGLOBAL_SET
000755301 980__ $$aEBOOK
000755301 980__ $$aBIB
000755301 982__ $$aEbook
000755301 983__ $$aOnline
000755301 994__ $$a92$$bISE