Field guidelines for genetic experimental designs in high-throughput sequencing [electronic resource] / Ana M. Aransay, José Luis Lavín Trueba, editors.
Aransay, Ana M., editor.; Trueba, José Luis Lavín, editor.
2016
QP625.N89
Available Online

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Title Field guidelines for genetic experimental designs in high-throughput sequencing [electronic resource] / Ana M. Aransay, José Luis Lavín Trueba, editors.
ISBN 9783319313504 (electronic book)
3319313509 (electronic book)
9783319313481
Published Switzerland : Springer, 2016.
Language English
Description 1 online resource (xi, 399 pages) : illustrations.
Call Number QP625.N89
Dewey Decimal Classification 572.8/633
Summary High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having such diversity of alternatives, there is a demand for information by research scientists without experience in HTS that need to choose the most suitable methodology or combination of platforms and to define their experimental designs to achieve their specific objectives. Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them. Moreover, examples of several successful strategies will be analyzed to make the point of the crucial features. This book will be of use to all scientist that are unfamiliar with HTS and want to incorporate such technologies to their research.
Note Includes index.
Bibliography, etc. Note Includes bibliographical references and index.
Access Note Access limited to authorized users.
Source of Description Online resource; title from PDF title page (SpringerLink, viewed June 15, 2016).
Added Author Aransay, Ana M., editor.
Trueba, José Luis Lavín, editor.
Available in Other Form Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing
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The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow
Whole genome sequencing recommendations
Counseling for targeted DNA region re-sequencing
Transcriptome profiling strategies
Differential mRNA Alternative Splicing
microRNA discovery and expression analysis in animals
Analysis of Long Non-coding RNAs in RNA-seq data
Ribosome profiling
Genome-wide analysis of DNA methylation patterns by high-throughput sequencing
Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments
PAR-CLIP: A genomic technique to dissect RNA-protein interactions
Metagenomic design and sequencing
A hitchhiker's guide to Metatranscriptomics
Eukaryotic Single-Cell mRNA Sequencing
Eukaryotic Single-Cell DNA Sequencing
Submitting data to a public repository, the final step of a successful HTS experiment.

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