Supervisor's Foreword; Acknowledgements; Contents; Abbreviations; Introduction; 1 Paper as a Substrate for Sensors; 1.1 Composition and Structure; 1.2 Advantages and Disadvantages of Paper as an Analytical Substrate; 1.3 Methods of Fabrication; 1.4 History of Paper in Analytics; 1.5 Paper-Based Analytics During the Last Decade; 1.5.1 Enhanced Sensitivity and Signal Amplification; 1.5.2 Three-Dimensional Architecture; 1.5.3 Timing and Valving; 1.5.4 Detection Methods; 1.5.4.1 Optical Detection; 1.5.4.2 Equipment Free Optical Detection; Electrochemical Detection; References

2 Analysis of Glucose, Cholesterol and Uric Acid2.1 Literature Review; 2.1.1 Importance of Glucose, Cholesterol and Uric Acid; 2.1.2 Methods of Quantification; 2.1.2.1 Common Detection Methods; Glucose; Uric Acid; Cholesterol; 2.1.2.2 Detection on Paper; Optical; Electrochemical; 2.1.3 Quantification of Proteins; 2.1.3.1 Clinical Significance of Determination of Protein Levels; 2.1.3.2 Detection of Proteins in Paper-Based Devices; 2.1.4 Enzyme Immobilization on Paper; 2.1.5 Flow in Paper Matrix; 2.1.5.1 Fluid Mechanics in Micro and Nano-Scale; 2.1.5.2 Flow in Paper Channel

2.1.5.3 Computer Modeling2.2 Materials and Methods; 2.2.1 Reagents and Materials; 2.2.2 Equipment; 2.2.3 Substrate; 2.2.3.1 Types of Paper; 2.2.3.2 Analysis of Paper Surface; 2.2.4 Analysis of Glucose, Cholesterol and Uric Acid; 2.2.4.1 Optical Detection; Fabrication of Prototype Devices; Choice of Colorimetric Method; Differences in Hue and Repeatability of Digitalization; Solving the Autoxidation Problem; New Fabrication Methods for Analysis of Cholesterol; Immobilization of the Colorimetric Reagent for Enhanced Uniformity of Color Development of Uric Acid Assay; Calibration Curves

Tangential Flow Devices2.2.4.2 Methods of Enzyme Immobilization on Paper; Protein Quantification Methods; Enzymatic Activity, Optimization of Assay Conditions; Enzyme Immobilization Methods; Impact of Enzyme Immobilization on Stability During Storage; Impact of Enzyme Immobilization on Flow in Paper Channel; Michaelis-Menten Kinetics; 2.2.4.3 Electrochemical Detection; Optimization of Device's Architecture; Analysis of Glucose and Uric Acid; Preliminary Tests; Preliminary Test Destined to Construct a Calibration Curve; Uniformity of Enzyme Immobilization; Electrode Positioning

Calibration Curve(Tutaj)Analysis of Glucose, Cholesterol and Uric Acid; Architecture; Conservation of Laminar Flow; Preliminary Tests and Optimization of the Architecture; 2.3 Results and Discussion; 2.3.1 Substrate; 2.3.2 Optical Detection; 2.3.2.1 Choice of Colorimetric Method; 2.3.2.2 Differences in Hue and Repeatability of Digitalization; 2.3.2.3 Solving the Autoxidation Problem; 2.3.2.4 New Fabrication Methods for Analysis of Cholesterol; 2.3.2.5 Immobilization of the Colorimetric Reagent for Enhanced Uniformity of Color Development of Uric Acid Assay; 2.3.2.6 Calibration Curves