000760188 000__ 04537cam\a2200493Ii\4500
000760188 001__ 760188
000760188 005__ 20230306142104.0
000760188 006__ m\\\\\o\\d\\\\\\\\
000760188 007__ cr\cn\nnnunnun
000760188 008__ 160829s2016\\\\sz\a\\\\o\\\\\001\0\eng\d
000760188 019__ $$a957597623
000760188 020__ $$a9783319350721$$q(electronic book)
000760188 020__ $$a3319350722$$q(electronic book)
000760188 020__ $$z9783319350707
000760188 020__ $$z3319350706
000760188 035__ $$aSP(OCoLC)ocn957464750
000760188 035__ $$aSP(OCoLC)957464750$$z(OCoLC)957597623
000760188 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dGW5XE$$dIDEBK$$dYDX$$dEBLCP
000760188 049__ $$aISEA
000760188 050_4 $$aQP552.M44
000760188 08204 $$a572/.696$$223
000760188 24504 $$aThe next generation in membrane protein structure determination$$h[electronic resource] /$$cIsabel Moraes, editor.
000760188 264_1 $$aSwitzerland :$$bSpringer,$$c2016.
000760188 300__ $$a1 online resource (x, 184 pages) :$$billustrations.
000760188 336__ $$atext$$btxt$$2rdacontent
000760188 337__ $$acomputer$$bc$$2rdamedia
000760188 338__ $$aonline resource$$bcr$$2rdacarrier
000760188 4901_ $$aAdvances in experimental medicine and biology,$$x0065-2598 ;$$vvolume 922
000760188 500__ $$aIncludes index.
000760188 5050_ $$aPreface; Acknowledgments; Contents; 1 Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters; 1.1 Introduction; 1.2 Bacteria; 1.3 Yeast; 1.4 Insect and Mammalian Cells; 1.5 Summary and Conclusions; References; 2 Detergents in Membrane Protein Purification and Crystallisation; 2.1 Physicochemical Properties of Detergents; 2.2 Role of Detergents in Membrane Protein Solubilisation; 2.3 Detergent Exchange or Removal; 2.4 Role of Detergents in Membrane Protein Stability; 2.5 Detergents for Membrane Protein Purification and Crystallisation
000760188 5058_ $$a2.5.1 Conventional Detergents2.5.2 New Detergents; 2.5.3 The Role of Detergents in Membrane Protein Crystallisation; 2.5.3.1 Mixed Micelles; 2.6 Conclusions; References; 3 NMR of Membrane Proteins: Beyond Crystals; 3.1 Introduction; 3.2 Barrel Folds; 3.3 Helical Targets; 3.4 Lipid Recognition Domains; 3.5 Protein Structure in Membranes; 3.6 Technologies; 3.7 DNP Sensitivity Enhancement; References; 4 Characterisation of Conformational and Ligand Binding Properties of Membrane Proteins Using Synchrotron Radiation Circular Dichroism (SRCD); 4.1 Introduction
000760188 5058_ $$a4.2 Diamond B23 Beamline for Synchrotron Radiation Circular Dichroism (SRCD)4.3 Ligand Binding Studies of the FsrC, Ace1 and SbmA Membrane Proteins; 4.4 Protein Stability; 4.5 Temperature Denaturation; 4.6 Conformational Analysis and HT-CD Screening of Protein Crystallisation Buffers; 4.7 Conclusion; References; 5 Membrane Protein Crystallisation: Current Trends and Future Perspectives; 5.1 Introduction; 5.2 Current Trends in the Number and Types of Alpha Helical Membrane Protein Structures; 5.3 Detergent Selection
000760188 5058_ $$a5.4 Precipitants -- How Do They Differ Between Membrane Proteins and Their Soluble Counterparts?5.5 MemGold and MemGold2 -- What's the Difference?; 5.6 Buffers, pH and Salts; 5.7 MemAdvantage -- An Alpha Helical Membrane Protein Additive Screen; 5.8 MemMeso -- A Systematically Designed in meso Crystallisation Screen; 5.9 Conclusions; References; 6 Crystal Dehydration in Membrane ProteinCrystallography; 6.1 Introduction; 6.2 The Dehydration Method; 6.2.1 Dehydration by Modifying the Vapour Equilibrium; 6.2.2 Dehydration by Modifying the Chemical Equilibrium
000760188 5058_ $$a6.2.3 Relative Humidity and Its Relationship with Cryo-Protection6.2.4 Crystal Changes Induced by Dehydration; 6.2.5 Effect of Dehydration on the Membrane-Protein Crystal Lattice; 6.3 Tools and Techniques for Crystal Dehydration; 6.3.1 Dehydration in the Laboratory; 6.3.1.1 Simple Air Exposure; 6.3.1.2 Vapour Control by Exchanging Well Solution; 6.3.1.3 Dehydration by Chemical Exchange of Crystallisation Droplet; 6.3.2 Modern Dehydration Methods with X-ray Feedback; 6.3.2.1 Capillary Dehydration; 6.3.2.2 Dehydration Using the FMS; 6.3.2.3 Dehydration Using the HC1 System
000760188 506__ $$aAccess limited to authorized users.
000760188 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed August 31, 2016).
000760188 650_0 $$aMembrane proteins.
000760188 7001_ $$aMoraes, Isabel,$$eeditor.
000760188 77608 $$iPrint version:$$z3319350706$$z9783319350707$$w(OCoLC)946461387
000760188 830_0 $$aAdvances in experimental medicine and biology ;$$vv. 922.
000760188 852__ $$bebk
000760188 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-319-35072-1$$zOnline Access$$91397441.1
000760188 909CO $$ooai:library.usi.edu:760188$$pGLOBAL_SET
000760188 980__ $$aEBOOK
000760188 980__ $$aBIB
000760188 982__ $$aEbook
000760188 983__ $$aOnline
000760188 994__ $$a92$$bISE