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Table of Contents
Preface; Contents; Part I: Lower Eukaryotes; 1: The Role ofßInorganic Polyphosphates inßStress Response andßRegulation ofßEnzyme Activities inßYeast; 1.1 Introduction; 1.2 Pi Limitation andßExcess; 1.3 Nitrogen Starvation; 1.4 Toxic Effects ofßManganese; 1.5 Adaptation toßHydrophobic Carbon Sources; 1.6 PolyP andßRegulation ofßEnzyme Activities; References; 2: Yeast Polyphosphatases PPX1 andßPPN1: Properties, Functions, andßLocalization; 2.1 Introduction; 2.2 Structural Features; 2.3 Substrate Specificity, Kinetics, Inhibitors andßActivators; 2.4 Cellular Localization.
2.5 Interplay ofßPPX1 andßPPN12.6 Cellular PolyP Under PPX1 andßPPN1 Knockout; 2.7 Cellular PolyP Under PPX1 andßPPN1 Overexpression; 2.8 Orthologous Proteins inßOther Organisms; References; 3: Polyphosphate Storage andßFunction inßAcidocalcisomes; 3.1 Introduction; 3.2 Polyphosphate Content ofßAcidocalcisomes; 3.3 Synthesis andßDegradation ofßAcidocalcisome PolyP; 3.4 Functions ofßAcidocalcisome PolyP; References; 4: Inorganic Polyphosphates inßMycorrhiza; 4.1 Mycorrhizal Symbiosis; 4.2 Identification ofßPolyphosphate inßMycorrhizal Fungi.
4.3 Technical Breakthroughs forßSpecific Detection ofßPolyphosphate4.4 Dynamics ofßPolyphosphate inßMycorrhizal Fungi; 4.5 Cellular Processes ofßPolyphosphate Accumulation; 4.6 Perspectives; References; 5: Functions ofßInositol Polyphosphate andßInorganic Polyphosphate inßYeast andßAmoeba; 5.1 Introduction; 5.2 Introduction toßInositol Polyphosphates; 5.3 Inositol Pyrophosphate Metabolism andßFunctions; 5.4 Inorganic Polyphosphate Eukaryote Metabolism; 5.5 Inositol Pyrophosphate andßInorganic Polyphosphate Metabolic Connected.
5.6 Inorganic Polyphosphate Function inßSaccharomyces cerevisiae5.7 Inorganic Polyphosphate Function inßDictyostelium discoideum; 5.8 Protein Polyphosphorylation; 5.9 Concluding Remarks; References; Part II: Higher Eukaryotes; 6: Methods ofßInorganic Polyphosphate (PolyP) Assay inßHigher Eukaryotic Cells; 6.1 Introduction; 6.2 Overview ofßtheßExperimental Approaches forßInvestigation ofßPolyP; 6.3 Extraction andßQuantification ofßPolyP inßEukaryotic Cells andßTissues; 6.4 Sizing ofßtheßExtracted PolyP; 6.5 Extraction andßAssessment ofßtheßComplexed PolyP.
6.6 Visualisation andßAssessment ofßPolyP inßLiving Cells6.7 Future Methods Development; References; 7: Inorganic Polyphosphates inßtheßMitochondria ofßMammalian Cells; 7.1 Introduction; 7.2 From Industry andßAgriculture toßMedicine: Sources ofßPolyPs inßMammalian Cells; 7.3 Pathways forßPolyP Synthesis andßDegradation; 7.4 Mitochondrial Metabolism ofßPolyP inßMammalian Cells; 7.5 Effects ofßPolyP Depletion onßtheßRespiratory Chain Activity; 7.6 Role ofßtheßInorganic PolyP inßMitochondrial Ca2+ Uptake andßCa2+ Buffering.
2.5 Interplay ofßPPX1 andßPPN12.6 Cellular PolyP Under PPX1 andßPPN1 Knockout; 2.7 Cellular PolyP Under PPX1 andßPPN1 Overexpression; 2.8 Orthologous Proteins inßOther Organisms; References; 3: Polyphosphate Storage andßFunction inßAcidocalcisomes; 3.1 Introduction; 3.2 Polyphosphate Content ofßAcidocalcisomes; 3.3 Synthesis andßDegradation ofßAcidocalcisome PolyP; 3.4 Functions ofßAcidocalcisome PolyP; References; 4: Inorganic Polyphosphates inßMycorrhiza; 4.1 Mycorrhizal Symbiosis; 4.2 Identification ofßPolyphosphate inßMycorrhizal Fungi.
4.3 Technical Breakthroughs forßSpecific Detection ofßPolyphosphate4.4 Dynamics ofßPolyphosphate inßMycorrhizal Fungi; 4.5 Cellular Processes ofßPolyphosphate Accumulation; 4.6 Perspectives; References; 5: Functions ofßInositol Polyphosphate andßInorganic Polyphosphate inßYeast andßAmoeba; 5.1 Introduction; 5.2 Introduction toßInositol Polyphosphates; 5.3 Inositol Pyrophosphate Metabolism andßFunctions; 5.4 Inorganic Polyphosphate Eukaryote Metabolism; 5.5 Inositol Pyrophosphate andßInorganic Polyphosphate Metabolic Connected.
5.6 Inorganic Polyphosphate Function inßSaccharomyces cerevisiae5.7 Inorganic Polyphosphate Function inßDictyostelium discoideum; 5.8 Protein Polyphosphorylation; 5.9 Concluding Remarks; References; Part II: Higher Eukaryotes; 6: Methods ofßInorganic Polyphosphate (PolyP) Assay inßHigher Eukaryotic Cells; 6.1 Introduction; 6.2 Overview ofßtheßExperimental Approaches forßInvestigation ofßPolyP; 6.3 Extraction andßQuantification ofßPolyP inßEukaryotic Cells andßTissues; 6.4 Sizing ofßtheßExtracted PolyP; 6.5 Extraction andßAssessment ofßtheßComplexed PolyP.
6.6 Visualisation andßAssessment ofßPolyP inßLiving Cells6.7 Future Methods Development; References; 7: Inorganic Polyphosphates inßtheßMitochondria ofßMammalian Cells; 7.1 Introduction; 7.2 From Industry andßAgriculture toßMedicine: Sources ofßPolyPs inßMammalian Cells; 7.3 Pathways forßPolyP Synthesis andßDegradation; 7.4 Mitochondrial Metabolism ofßPolyP inßMammalian Cells; 7.5 Effects ofßPolyP Depletion onßtheßRespiratory Chain Activity; 7.6 Role ofßtheßInorganic PolyP inßMitochondrial Ca2+ Uptake andßCa2+ Buffering.