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Preface; Contents; Chapter 1: Monitoring and Modeling Algal Blooms; 1.1 Monitoring of Cyanobacteria; 1.1.1 Microscopy; 1.1.1.1 Advantages and Limitations; 1.1.2 Online Phycocyanin Probe; 1.1.2.1 Advantages and Limitations; 1.1.3 Conventional Molecular Techniques; 1.1.3.1 Polymerase Chain Reaction (PCR) and Real-Time Quantitative PCR (qPCR); The PCR Process Consists of Three Steps; The PCR Process Can Be Divided into Three Stages; Advantages and Limitations of PCR and qPCR; 1.1.3.2 Fluorescence In Situ Hybridization (FISH); FISH Procedure Includes the Following Steps Shown in Fig.1.1

Advantages and Limitations of FISH1.1.4 High-Throughput Sequencing (HTS); 1.1.4.1 Advantages and Limitations; 1.2 Modeling of Algal Blooms; 1.2.1 Artificial Neural Network (ANN); 1.2.1.1 Advantages and Limitations; Black Box; Long Computing Time; Overfitting; 1.2.2 Support Vector Machine (SVM); 1.2.2.1 Advantages and Limitations; 1.2.3 Relevance Vector Machine (RVM); 1.2.3.1 Advantages and Limitations; 1.2.4 Extreme Learning Machine (ELM); 1.2.4.1 Advantages and Limitations; 1.3 Conclusions; References; Chapter 2: Water Resources and Importance of Reservoirs; 2.1 Water Resource Situation

2.1.1 Raw Water Imported from China2.1.2 Reservoirs; 2.1.3 Water Reuse; 2.1.4 Water-Saving Policies; 2.2 Importance of Reservoirs; 2.2.1 Salty Periods; 2.2.2 Environmental Pollution; 2.3 Eutrophication Analysis; Reference; Chapter 3: Spatiotemporal Variations of Phytoplankton Structure and Water Quality in the Eutrophic Freshwater Reservoir of Macau; 3.1 Introduction; 3.2 Materials and Methods; 3.2.1 Site Description and Sampling; 3.2.2 Water Quality Parameters; 3.2.2.1 Abiotic Parameters; 3.2.2.2 Biotic Parameters; 3.2.3 Data Analysis; 3.2.3.1 Trophic State Index (TSI)

3.2.3.2 Community Comparison Indices3.2.3.3 Statistical Analysis; 3.2.4 Principal Component Analysis (PCA); 3.2.5 Cluster Analysis (CA); 3.2.6 Discriminant Analysis (DA); 3.3 Results; 3.3.1 Water Quality in MSR; 3.3.1.1 Abiotic Parameters; 3.3.2 MSR Phytoplankton Community; 3.3.2.1 Phytoplankton Species Compositions and Densities; 3.3.2.2 Diversity, Dominance, Evenness, Richness, and Similarity; 3.3.3 Principal Component Analysis (PCA); 3.3.4 Temporal Variations; 3.3.4.1 Cluster Analysis (CA); 3.3.4.2 Discriminant Analysis (DA); 3.3.5 Spatial Variations; 3.3.5.1 Cluster Analysis (CA)

3.3.5.2 Discriminant Analysis (DA)3.4 Discussion; 3.5 Conclusions; References; Chapter 4: Using an Online Phycocyanin Fluorescence Probe for Rapid Monitoring of Cyanobacteria in Macau Freshwater Reservoir; 4.1 Introduction; 4.2 Materials and Methods; 4.2.1 PC Probe; 4.2.2 Unicellular Culture Cultivation; 4.2.3 MSR and Sampling; 4.2.4 Enumeration of Cyanobacteria; 4.2.5 Measurement of Phycocyanin, Cylindrospermopsin, and Chl-a; 4.3 Results; 4.3.1 Biovolume; 4.3.2 Unicellular Pure Cultures; 4.3.2.1 Detection Limit of the Probe; 4.3.2.2 Mixed Cultures; 4.3.3 Water Samples; 4.4 Discussion

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