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Dedication; Foreword; Preface; Contents; Contributors; About the Editors; 1: Introduction to Chromosome; 1.1 Introductory Remarks; 1.2 Early History of Chromosome; 1.3 Chemical Nature of Chromosome; 1.4 Chromosome Packaging; 1.5 Chromosomal Aberrations; 1.6 Artificial Chromosomes; 1.7 Chromosome Painting; Glossary; References and Additional Reading; 2: B Chromosomes; 2.1 Introduction; 2.2 B Chromosome Inheritance Mechanisms; 2.3 Origins and Sequence Content of B Chromosomes; 2.4 What Are B Chromosomes Doing?; 2.5 Applications for B Chromosomes; 2.6 Conclusions; Glossary
4.3.6 Acetic Acid (CH3COOH)4.3.7 Formaldehyde (HCHO); 4.3.8 Propionic Acid (C2H5COOH); 4.3.9 Chloroform (CHCl3); 4.4 Fixing Mixtures; 4.4.1 Flemming's Weak Fluid (1882) and Flemming's Strong Fluid (1884); 4.4.2 Carnoy's Fluid (1886); 4.4.3 Carnoy's Fluid II (1886); 4.4.4 Navaschin's Fluid (Navaschin 1925); 4.5 Stain and Staining; 4.5.1 Carmine; 4.5.2 Orcein; 4.5.3 Crystal Violet (Methyl Violet 10B); 4.5.4 Hematoxylin; 4.5.5 Fuchsine (Fuschin); 4.5.5.1 The Feulgen Reaction; 4.6 Preparations of Some of the Important Stains Used in Squash and Smear Techniques; 4.6.1 Acetocarmine, Aceto-orcein
4.6.2 Crystal Violet4.6.3 Schiff's Reagent/Fuchsine Reagent; 4.7 Squash and Smear Preparation; 4.7.1 Smear Method; 4.7.2 Squash Method; 4.7.3 Making Slides Permanent; References; 5: Flow Cytometry and Its Utility; 5.1 Introduction; 5.2 Flow Cytometric Measurement; 5.2.1 Absorbance and Scattering Monitoring; 5.2.2 Fluorescence Monitoring; 5.2.3 Probes; 5.3 Utility of Flow Cytometry; 5.3.1 Cell Size, Shape, and Granularity; 5.3.2 Viability and Apoptosis; 5.3.3 Nuclear DNA Content, Cell Cycle, and Ploidy; 5.3.4 Flow Karyotyping and Chromosome Sorting; 5.3.4.1 Chromosome Aberrations
4.3.6 Acetic Acid (CH3COOH)4.3.7 Formaldehyde (HCHO); 4.3.8 Propionic Acid (C2H5COOH); 4.3.9 Chloroform (CHCl3); 4.4 Fixing Mixtures; 4.4.1 Flemming's Weak Fluid (1882) and Flemming's Strong Fluid (1884); 4.4.2 Carnoy's Fluid (1886); 4.4.3 Carnoy's Fluid II (1886); 4.4.4 Navaschin's Fluid (Navaschin 1925); 4.5 Stain and Staining; 4.5.1 Carmine; 4.5.2 Orcein; 4.5.3 Crystal Violet (Methyl Violet 10B); 4.5.4 Hematoxylin; 4.5.5 Fuchsine (Fuschin); 4.5.5.1 The Feulgen Reaction; 4.6 Preparations of Some of the Important Stains Used in Squash and Smear Techniques; 4.6.1 Acetocarmine, Aceto-orcein
4.6.2 Crystal Violet4.6.3 Schiff's Reagent/Fuchsine Reagent; 4.7 Squash and Smear Preparation; 4.7.1 Smear Method; 4.7.2 Squash Method; 4.7.3 Making Slides Permanent; References; 5: Flow Cytometry and Its Utility; 5.1 Introduction; 5.2 Flow Cytometric Measurement; 5.2.1 Absorbance and Scattering Monitoring; 5.2.2 Fluorescence Monitoring; 5.2.3 Probes; 5.3 Utility of Flow Cytometry; 5.3.1 Cell Size, Shape, and Granularity; 5.3.2 Viability and Apoptosis; 5.3.3 Nuclear DNA Content, Cell Cycle, and Ploidy; 5.3.4 Flow Karyotyping and Chromosome Sorting; 5.3.4.1 Chromosome Aberrations