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Applications of aminoacylation ribozymes that recognize the 3′-end of tRNA / Naohiro Terasaka.

Terasaka, Naohiro, author.
2017
QP619.A45
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Title
Applications of aminoacylation ribozymes that recognize the 3′-end of tRNA / Naohiro Terasaka.
Author
Terasaka, Naohiro, author.
ISBN
9784431565178 (electronic book)
4431565175 (electronic book)
9784431565154
DOI
https://doi.org/10.1007/978-4-431-56517-8
Published
Tokyo : Springer, [2017]
Copyright
©2017
Language
English
Description
1 online resource.
Item Number
10.1007/978-4-431-56517-8 doi
Call Number
QP619.A45
Dewey Decimal Classification
572.886
Summary
In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery. The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.
Dissertation Note
Thesis (Ph. D.)--University of Tokyo.
Bibliography, etc. Note
Includes bibliographical references and index.
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Access limited to authorized users.
Digital File Characteristics
text file PDF
Source of Description
Online resource; title from PDF title page (viewed March 21, 2017).
Series
Springer theses.
Available in Other Form
Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA
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Supervisor's Foreword; Parts of this thesis have been published in the following journal articles; Acknowledgements; Contents; 1 General Introduction; Abstract; 1.1 Transfer RNA; 1.2 Other Noncoding RNAs Than tRNAs; 1.3 Aminoacylation Ribozyme "Flexizyme"; References; 2 Discovery of Human MicroRNA Precursor Binding to Folic Acid by Small RNA Transcriptomic SELEX; Abstract; 2.1 Introduction; 2.2 Results and Discussions; 2.2.1 Summary of Master Course Study; 2.2.2 KD Determination and Mutation Study Using Biolayer Interferometry; 2.3 Conclusion; 2.4 Materials and Methods; References

3 Orthogonal Ribosome-tRNAs Pair by Engineering of Peptidyl Transferase CenterAbstract; 3.1 Introduction; 3.2 Results and Discussions; 3.2.1 Aminoacylation of CCA-Mutated tRNAs; 3.2.2 Preparation of Tagged Ribosomes; 3.2.3 Translation Activity of Wild-type/Mutant Ribosome-tRNA Pairs; 3.2.4 Simultaneous Expression of Two Different Peptides from Single mRNA Template; 3.3 Conclusion; 3.4 Materials and Methods; References; 4 General Conclusion; Abstract; 4.1 General Conclusion; References; Curriculum Vitae

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