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Foreword; Preface; Contents; About the Authors; 1 Nucleotide Analysis; 1.1 Sequence Retrieval; 1.2 Primer Designing; 1.3 Designing Degenerate Primers; 1.4 Reading of Sequence Trace Files Using Finch TV; 1.5 Troubleshooting DNA Sequencing Problems; 1.6 Editing Sequence Data; 1.7 Sequence Assembly-CAP3 Program; 1.8 Checking for Vector Contamination; 1.9 Restriction Mapping Using NEBcutter; 1.10 Gene Prediction Using ORF Finder (Open Reading Frame Finder); 1.11 Gene Prediction Using FGENESB; 1.12 Dot-Plot; 1.13 Global Sequence Alignment; 1.14 Local Sequence Alignment
1.15 Basic Local Alignment Search Tool (BLAST)-Nucleotide BLAST1.16 Interpreting BLAST Result; 1.17 Multiple Sequence Alignment: T-Coffee for Small Alignments; 1.18 Multiple Sequence Alignment-MUSCLE for Medium Alignments; 1.19 Multiple Sequence Alignment-MAFFT for Large Alignments; 1.20 Multiple Sequence Alignment and Phylogenetic Analysis Using MEGA; 2 DNA Marker Analysis; 2.1 Genetic Analysis Using NTSYSpc (Numerical Taxonomy System); 2.2 Principal Coordinate Analysis (PCOORDA) Using NTSYSpc; 2.3 Population Genetic Analysis Using PowerMarker
2.4 Dissimilarity Analysis-DARwin5 (Dissimilarity Analysis and Representation for Windows)3 RNA Analysis; 3.1 Predicting RNA Secondary Structure; 3.2 Finding Repeats; 4 Protein Sequence Analysis; 4.1 Protein Sequence Retrieval from UniProtKB; 4.2 Visualization of Features in a Multiple Sequence Alignment; 4.3 Predicting Signal Peptides in Proteins Using SignalP 4.1 Server; 4.4 Predicting Transmembrane Segments and Signal Peptides in Proteins Using Phobius; 4.5 Predicting Subcellular Location Using TargetP; 4.6 Protein BLAST (blastp); 4.7 Position-Specific Iterated (PSI)-BLAST
4.8 Creating Pattern from Alignment4.9 Pattern-Hit Initiated (PHI)-BLAST; 4.10 Domain Enhanced Lookup Time Accelerated-BLAST (DELTA-BLAST); 5 Protein Structure Analysis; 5.1 Protein Primary Structure Analysis-ProtParam; 5.2 Protein Secondary Structure Prediction; 5.2.1 Secondary Structure Prediction Using SOPMA; 5.2.2 Secondary Structure Prediction Using PSIPRED; 5.3 Protein Tertiary Structure Prediction by Homology Modelling; 5.3.1 Homology Modelling Using SwissModel; 5.3.2 Protein Tertiary Structure Prediction by Threading (Fold Recognition); 5.4 Protein Tertiary Structure Analysis
5.4.1 RAMPAGE5.4.2 Protein Structure Analysis Using SAVeS; 5.5 Protein Structure Visualization; 5.5.1 RasMol; 5.5.2 PyMol; 5.6 Protein Structure Alignment/Superimpose Using SuperPose; 5.7 Protein Cleft Analysis; 6 Protein-Ligand Interactions; 6.1 Protein-Ligand Docking Using AutoDock4.1 and MGLTools; 6.2 Protein-Protein Docking Using ClusPro2.0; Appendix: Online Resources on Bioinformatics; References
1.15 Basic Local Alignment Search Tool (BLAST)-Nucleotide BLAST1.16 Interpreting BLAST Result; 1.17 Multiple Sequence Alignment: T-Coffee for Small Alignments; 1.18 Multiple Sequence Alignment-MUSCLE for Medium Alignments; 1.19 Multiple Sequence Alignment-MAFFT for Large Alignments; 1.20 Multiple Sequence Alignment and Phylogenetic Analysis Using MEGA; 2 DNA Marker Analysis; 2.1 Genetic Analysis Using NTSYSpc (Numerical Taxonomy System); 2.2 Principal Coordinate Analysis (PCOORDA) Using NTSYSpc; 2.3 Population Genetic Analysis Using PowerMarker
2.4 Dissimilarity Analysis-DARwin5 (Dissimilarity Analysis and Representation for Windows)3 RNA Analysis; 3.1 Predicting RNA Secondary Structure; 3.2 Finding Repeats; 4 Protein Sequence Analysis; 4.1 Protein Sequence Retrieval from UniProtKB; 4.2 Visualization of Features in a Multiple Sequence Alignment; 4.3 Predicting Signal Peptides in Proteins Using SignalP 4.1 Server; 4.4 Predicting Transmembrane Segments and Signal Peptides in Proteins Using Phobius; 4.5 Predicting Subcellular Location Using TargetP; 4.6 Protein BLAST (blastp); 4.7 Position-Specific Iterated (PSI)-BLAST
4.8 Creating Pattern from Alignment4.9 Pattern-Hit Initiated (PHI)-BLAST; 4.10 Domain Enhanced Lookup Time Accelerated-BLAST (DELTA-BLAST); 5 Protein Structure Analysis; 5.1 Protein Primary Structure Analysis-ProtParam; 5.2 Protein Secondary Structure Prediction; 5.2.1 Secondary Structure Prediction Using SOPMA; 5.2.2 Secondary Structure Prediction Using PSIPRED; 5.3 Protein Tertiary Structure Prediction by Homology Modelling; 5.3.1 Homology Modelling Using SwissModel; 5.3.2 Protein Tertiary Structure Prediction by Threading (Fold Recognition); 5.4 Protein Tertiary Structure Analysis
5.4.1 RAMPAGE5.4.2 Protein Structure Analysis Using SAVeS; 5.5 Protein Structure Visualization; 5.5.1 RasMol; 5.5.2 PyMol; 5.6 Protein Structure Alignment/Superimpose Using SuperPose; 5.7 Protein Cleft Analysis; 6 Protein-Ligand Interactions; 6.1 Protein-Ligand Docking Using AutoDock4.1 and MGLTools; 6.2 Protein-Protein Docking Using ClusPro2.0; Appendix: Online Resources on Bioinformatics; References