Linked e-resources
Details
Table of Contents
Editors' Biographies; Part I Introduction; 1 Pollen Tubes and Tip Growth: of Biophysics and Tipomics; 1.1 Introduction; 1.2 Some Unsolved (Bio)physical Aspects of Tip Growth; 1.3 Technical Improvements and a Wish List for the Future; 1.4 Tipomics: Pollen Systems Biology and Modelling Tip Growth; 1.5 Conclusion, Open Questions and Perspective; References; Part II Biophysics of Tip Growth; 2 Water Transport in Pollen; Abbreviations; 2.1 Introduction; 2.2 Water Potential, Water Transport and Pollen Tube Growth; 2.3 Measuring Water Potential Parameters and Water Transport in Pollen
2.3.1 Protoplast Swell Assays2.3.2 Pressure Probe Technique; 2.4 Pollen Water Transport; 2.4.1 Aquaporins in Pollen; 2.4.2 Pollen Grain Rehydration; 2.4.3 Pollen Tube Elongation; 2.5 Osmosensing and Osmoregulation in Pollen; 2.6 Challenges for the Future: Reproduction and Drought Stress; 2.7 Conclusion and Perspective; References; 3 The Cytoskeleton of Pollen Tubes and How It Determines the Physico-mechanical Properties of Cell Wall; Abbreviations; 3.1 Introduction; 3.2 The Cytoskeleton of Pollen Tubes; 3.2.1 Actin Filaments
3.2.2 Actin-Binding Proteins and Actin-Based Motors: How Do They Affect Cell Wall Synthesis?3.2.3 Microtubules; 3.2.4 Microtubule-Associated Proteins that May Take Part in Cell Wall Deposition; 3.3 Synthesis and Deposition of the Cell Wall in Pollen Tubes; 3.3.1 Secretion of Cell Wall Components: Pectins, Arabinogalactan Proteins, and the Cytoskeleton; 3.3.2 Plasma Membrane-Localized Synthesis of Cell Wall Polysaccharides; 3.3.3 Cytoskeleton-Based Delivery of Glucan Synthases; 3.4 Regulation of Cell Wall Synthesis Via Sucrose-Metabolizing Enzymes
3.5 How the Cytoskeleton Affects the Physicochemical Properties of the Cell Wall3.6 Conclusion and Perspective; References; Part III Technical Improvements to Study Tip Growth; 4 Measuring Cytomechanical Forces on Growing Pollen Tubes; Abbreviations; 4.1 Introduction; 4.2 How Are Cytomechanical Parameters Measured?; 4.2.1 Measuring Turgor; 4.2.2 Measuring Penetration Forces; 4.2.3 Stiffness and Topography Mapping-Indentation Methods; 4.3 Cellular Force Microscopy; 4.3.1 General Considerations; 4.3.2 CFM Measurements on Pollen Tubes; 4.3.3 Limitations of the CFM Approach; 4.4 Improvements
4.4.1 Microchannel Guidance4.4.2 Real-Time Computer Vision; 4.4.3 Dual-Axis Force Sensors; 4.5 Conclusions and Further Developments; References; 5 Microfluidic- and Microelectromechanical System (MEMS)-Based Platforms for Experimental Analysis of Pollen Tube Growth Behavior and Quantification of Cell Mechanical Properties; Abbreviations; 5.1 Introduction; 5.2 Design Principles and Considerations; 5.2.1 Basic Design and Fabrication; 5.2.2 Dimensions and Features of the Microfluidic Network; 5.2.3 Influence of Microchannel Geometry and Fluid Flow on Pollen Tube Performance
2.3.1 Protoplast Swell Assays2.3.2 Pressure Probe Technique; 2.4 Pollen Water Transport; 2.4.1 Aquaporins in Pollen; 2.4.2 Pollen Grain Rehydration; 2.4.3 Pollen Tube Elongation; 2.5 Osmosensing and Osmoregulation in Pollen; 2.6 Challenges for the Future: Reproduction and Drought Stress; 2.7 Conclusion and Perspective; References; 3 The Cytoskeleton of Pollen Tubes and How It Determines the Physico-mechanical Properties of Cell Wall; Abbreviations; 3.1 Introduction; 3.2 The Cytoskeleton of Pollen Tubes; 3.2.1 Actin Filaments
3.2.2 Actin-Binding Proteins and Actin-Based Motors: How Do They Affect Cell Wall Synthesis?3.2.3 Microtubules; 3.2.4 Microtubule-Associated Proteins that May Take Part in Cell Wall Deposition; 3.3 Synthesis and Deposition of the Cell Wall in Pollen Tubes; 3.3.1 Secretion of Cell Wall Components: Pectins, Arabinogalactan Proteins, and the Cytoskeleton; 3.3.2 Plasma Membrane-Localized Synthesis of Cell Wall Polysaccharides; 3.3.3 Cytoskeleton-Based Delivery of Glucan Synthases; 3.4 Regulation of Cell Wall Synthesis Via Sucrose-Metabolizing Enzymes
3.5 How the Cytoskeleton Affects the Physicochemical Properties of the Cell Wall3.6 Conclusion and Perspective; References; Part III Technical Improvements to Study Tip Growth; 4 Measuring Cytomechanical Forces on Growing Pollen Tubes; Abbreviations; 4.1 Introduction; 4.2 How Are Cytomechanical Parameters Measured?; 4.2.1 Measuring Turgor; 4.2.2 Measuring Penetration Forces; 4.2.3 Stiffness and Topography Mapping-Indentation Methods; 4.3 Cellular Force Microscopy; 4.3.1 General Considerations; 4.3.2 CFM Measurements on Pollen Tubes; 4.3.3 Limitations of the CFM Approach; 4.4 Improvements
4.4.1 Microchannel Guidance4.4.2 Real-Time Computer Vision; 4.4.3 Dual-Axis Force Sensors; 4.5 Conclusions and Further Developments; References; 5 Microfluidic- and Microelectromechanical System (MEMS)-Based Platforms for Experimental Analysis of Pollen Tube Growth Behavior and Quantification of Cell Mechanical Properties; Abbreviations; 5.1 Introduction; 5.2 Design Principles and Considerations; 5.2.1 Basic Design and Fabrication; 5.2.2 Dimensions and Features of the Microfluidic Network; 5.2.3 Influence of Microchannel Geometry and Fluid Flow on Pollen Tube Performance