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Preface; Contents; About the Author; Chapter 1: The Basic Concept of Microbiology; 1.1 Aseptic Technique and Safety; 1.2 Good Microbiological Laboratory Practices (GMLP); 1.3 Risk Assessment; 1.4 Spillage Management; 1.4.1 Spills; 1.4.2 Broken Glass; 1.4.3 Splashes on Clothing and the Skin; 1.5 Resources; 1.5.1 Equipment (Source: ASE 2001); 1.5.2 Apparatus (Source: ASE 2001); 1.5.3 Materials (Source: ASE 2001); 1.6 Flaming the Needle/Loop; 1.7 Flaming the Neck of Bottles/Test Tubes; 1.8 Media; 1.8.1 Preparation of Culture Media; 1.8.2 Pouring a Petri Plate; 1.8.3 Agar Slants
1.8.4 Storage of Prepared Media1.9 Disinfectants; 1.10 Incubation; 1.11 Accidents; 1.12 Emergency Preparation and Response; References; Chapter 2: Collection and Processing for Trichoderma Specimen; 2.1 Introduction; 2.2 Media for Isolating of Trichoderma; 2.2.1 Trichoderma Selective Media; 2.2.2 T. harzianum-Selective Medium (THSM); 2.2.3 Rose Bengal Agar (RBA); 2.3 Materials; 2.4 Methods; 2.4.1 Sample Collection Procedures; 2.4.2 Isolation of Trichoderma Colony; 2.4.3 Sample Dilution; 2.4.4 Culturing Plates; 2.4.5 Preparation of Slant Media; 2.4.6 Slant Culture
2.5 Identification of Trichoderma Isolates2.6 Pure Culture of Trichoderma Isolates; 2.7 Direct Culture Methods; 2.8 Storage of Trichoderma; 2.8.1 Storage in Water; 2.8.2 Cold Temperature; 2.8.3 Under Mineral Oil; 2.8.4 Freeze Drying or Lyophilization; 2.8.5 Low-Temperature Storage; References; Chapter 3: Slide Culturing of Trichoderma Isolates; 3.1 Introduction; 3.2 Materials; 3.3 Procedure; 3.3.1 Slide Culture Technique I; 3.3.2 Slide Culture Technique II; Chapter 4: Morphology-Based Characterization of Trichoderma Species; 4.1 Introduction
4.2 Definitions and Taxonomic Value of Morphological Traits4.2.1 Teleomorph; 4.2.2 Cultures; 4.2.3 Growth Media; 4.2.4 Growth Plates/Colonies; 4.2.5 Growth Plates/Conidiation; 4.2.6 Conidial Heads, Wet Versus Dry; 4.2.7 Conidiophores/Trees, Regular; 4.2.8 Conidiophore Terminology (Source: Jaklitsch 2009); 4.2.9 Effuse Conidiation (Synanamorphs); 4.2.10 Pustules; 4.2.11 Shrubs, Granules; 4.2.12 Tufts, Also Called "Fluffy Tufts"; 4.3 Macroscopic Analysis; 4.3.1 Trichoderma asperellum; 4.3.2 Trichoderma harzianum; 4.3.3 Trichoderma hamatum; 4.3.4 Trichoderma reesei
4.3.5 Trichoderma longibrachiatum4.3.6 Trichoderma spirale; 4.3.7 Trichoderma brevicompactum; 4.3.8 Trichoderma erinaceum; 4.3.9 Trichoderma koningiopis; 4.3.10 Trichoderma melanomagna; 4.3.11 Trichoderma viride; 4.3.12 Trichoderma theobromicola; References; Chapter 5: Molecular Characteristics of Trichoderma Strains; 5.1 Molecular Analysis; 5.2 DNA Extraction and Purification; 5.3 Obtaining Materials from Which to Extract DNA; 5.3.1 Trichoderma Culture; 5.3.2 Trichoderma Liquid Culture; 5.4 DNA Extraction Protocols; 5.4.1 Phenol-Chloroform Method
1.8.4 Storage of Prepared Media1.9 Disinfectants; 1.10 Incubation; 1.11 Accidents; 1.12 Emergency Preparation and Response; References; Chapter 2: Collection and Processing for Trichoderma Specimen; 2.1 Introduction; 2.2 Media for Isolating of Trichoderma; 2.2.1 Trichoderma Selective Media; 2.2.2 T. harzianum-Selective Medium (THSM); 2.2.3 Rose Bengal Agar (RBA); 2.3 Materials; 2.4 Methods; 2.4.1 Sample Collection Procedures; 2.4.2 Isolation of Trichoderma Colony; 2.4.3 Sample Dilution; 2.4.4 Culturing Plates; 2.4.5 Preparation of Slant Media; 2.4.6 Slant Culture
2.5 Identification of Trichoderma Isolates2.6 Pure Culture of Trichoderma Isolates; 2.7 Direct Culture Methods; 2.8 Storage of Trichoderma; 2.8.1 Storage in Water; 2.8.2 Cold Temperature; 2.8.3 Under Mineral Oil; 2.8.4 Freeze Drying or Lyophilization; 2.8.5 Low-Temperature Storage; References; Chapter 3: Slide Culturing of Trichoderma Isolates; 3.1 Introduction; 3.2 Materials; 3.3 Procedure; 3.3.1 Slide Culture Technique I; 3.3.2 Slide Culture Technique II; Chapter 4: Morphology-Based Characterization of Trichoderma Species; 4.1 Introduction
4.2 Definitions and Taxonomic Value of Morphological Traits4.2.1 Teleomorph; 4.2.2 Cultures; 4.2.3 Growth Media; 4.2.4 Growth Plates/Colonies; 4.2.5 Growth Plates/Conidiation; 4.2.6 Conidial Heads, Wet Versus Dry; 4.2.7 Conidiophores/Trees, Regular; 4.2.8 Conidiophore Terminology (Source: Jaklitsch 2009); 4.2.9 Effuse Conidiation (Synanamorphs); 4.2.10 Pustules; 4.2.11 Shrubs, Granules; 4.2.12 Tufts, Also Called "Fluffy Tufts"; 4.3 Macroscopic Analysis; 4.3.1 Trichoderma asperellum; 4.3.2 Trichoderma harzianum; 4.3.3 Trichoderma hamatum; 4.3.4 Trichoderma reesei
4.3.5 Trichoderma longibrachiatum4.3.6 Trichoderma spirale; 4.3.7 Trichoderma brevicompactum; 4.3.8 Trichoderma erinaceum; 4.3.9 Trichoderma koningiopis; 4.3.10 Trichoderma melanomagna; 4.3.11 Trichoderma viride; 4.3.12 Trichoderma theobromicola; References; Chapter 5: Molecular Characteristics of Trichoderma Strains; 5.1 Molecular Analysis; 5.2 DNA Extraction and Purification; 5.3 Obtaining Materials from Which to Extract DNA; 5.3.1 Trichoderma Culture; 5.3.2 Trichoderma Liquid Culture; 5.4 DNA Extraction Protocols; 5.4.1 Phenol-Chloroform Method