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Acknowledgements; Funding Information; Contents; 1: Introduction; References; 2: Harvest, Transport ofßSpawners, Prophylaxis; Harvesting ofßSpawners; Transporting ofßSpawners; Prophylaxis; References; 3: Hatchery Manipulation andßBroodstock Selection; Theoretical Background; Conditions forßKeeping theßSpawners; Water Quality Parameters; Sex Recognition; Hatchery Manipulations; Basic Information onßAnesthesia; Practical Tips; Anesthesia; Sex Recognition; References; 4: Determination ofßMaturity Stages ofßOocytes; Theoretical Background; Maturation Stages ofßOocytes.
Specific Cases ofßtheßFOM Disruption Anomalies Before theßGVBD; Anomalies After theßGVBD; Practical Evaluation ofßtheßMaturity Stage ofßtheßFemales; References; 5: Stimulation ofßOvulation andßSpermiation; Rationale ofßtheßApplication ofßHormonal Stimulation; Photo-Thermal Manipulations; Theoretical Background ofßHormonal Stimulation; Regulations onßtheßUsage ofßSpawning Agents inßEurope; Where toßInject theßFish?; Hormonal Stimulation ofßFemales; Preparation ofßtheßHormonal Agentß- hCG; Preparation ofßtheßHormonal Agentß- GnRH; Hormonal Stimulation ofßMales; Practical Tips.
Photo-Thermal Manipulations Hormonal Stimulation; References; 6: Collection of Gametes; Characteristics of Gametes; Determination of the Moment of Ovulation; Prevention of Spontaneous Ovulation; Recognition of the Moment of Ovulation; Collection of Eggs; Collection of Sperm; References; 7: Short- and Long-Term Storage of Gametes; Rationale of Gamete Storage; Short-Term Storage; Sperm Cryopreservation; Short Term Storage of Eggs of Eurasian Perch; Short-Term Storage of Sperm; Cryopreservation and Thawing; References; 8: Evaluation of Gamete Quality; How to Define Gamete Quality?
Methods ofßEvaluation ofßGamete Quality Hatchery-Related Factors Affecting Gamete Quality; Evaluation ofßtheßEgg Quality; Morphological Evaluation Off Egg Quality; Fragmentation ofßOil Droplets; Morphological Alterations ofßVarious Types; First Cleavages andßBlastomere Morphology; Practical Tips onßEvaluation ofßEgg Quality; Embryonic Survival Rate; Evaluation ofßtheßSperm Quality by theßMotility Assessment; References; 9: In Vitro Fertilization; Theoretical Background; Technique ofßIn Vitro Fertilization; Activation ofßtheßGametes; Activating Solutions; Sperm-to-Egg Ratio.
Practical Aspects ofßIn Vitro FertilizationReferences; 10: Incubation andßHatching; Theoretical Background; Embryonic Development; Verification ofßtheßEffectiveness ofßFertilization; Incubation Devices; Incubation Conditions; Hatching; Initial Larvae Rearing (General Advices); References; 11: Advanced Spawning (Out-of theßSeason Spawning); Theoretical Background; State-of-the-Art ofß'Advanced Spawning' inßtheßEurasian Perch; Practical Recommendations; Broodstock Management; Photo-Thermal Manipulation; Stimulation ofßOvulation andßSpermiation; References; Appendices; Appendix 1.
Specific Cases ofßtheßFOM Disruption Anomalies Before theßGVBD; Anomalies After theßGVBD; Practical Evaluation ofßtheßMaturity Stage ofßtheßFemales; References; 5: Stimulation ofßOvulation andßSpermiation; Rationale ofßtheßApplication ofßHormonal Stimulation; Photo-Thermal Manipulations; Theoretical Background ofßHormonal Stimulation; Regulations onßtheßUsage ofßSpawning Agents inßEurope; Where toßInject theßFish?; Hormonal Stimulation ofßFemales; Preparation ofßtheßHormonal Agentß- hCG; Preparation ofßtheßHormonal Agentß- GnRH; Hormonal Stimulation ofßMales; Practical Tips.
Photo-Thermal Manipulations Hormonal Stimulation; References; 6: Collection of Gametes; Characteristics of Gametes; Determination of the Moment of Ovulation; Prevention of Spontaneous Ovulation; Recognition of the Moment of Ovulation; Collection of Eggs; Collection of Sperm; References; 7: Short- and Long-Term Storage of Gametes; Rationale of Gamete Storage; Short-Term Storage; Sperm Cryopreservation; Short Term Storage of Eggs of Eurasian Perch; Short-Term Storage of Sperm; Cryopreservation and Thawing; References; 8: Evaluation of Gamete Quality; How to Define Gamete Quality?
Methods ofßEvaluation ofßGamete Quality Hatchery-Related Factors Affecting Gamete Quality; Evaluation ofßtheßEgg Quality; Morphological Evaluation Off Egg Quality; Fragmentation ofßOil Droplets; Morphological Alterations ofßVarious Types; First Cleavages andßBlastomere Morphology; Practical Tips onßEvaluation ofßEgg Quality; Embryonic Survival Rate; Evaluation ofßtheßSperm Quality by theßMotility Assessment; References; 9: In Vitro Fertilization; Theoretical Background; Technique ofßIn Vitro Fertilization; Activation ofßtheßGametes; Activating Solutions; Sperm-to-Egg Ratio.
Practical Aspects ofßIn Vitro FertilizationReferences; 10: Incubation andßHatching; Theoretical Background; Embryonic Development; Verification ofßtheßEffectiveness ofßFertilization; Incubation Devices; Incubation Conditions; Hatching; Initial Larvae Rearing (General Advices); References; 11: Advanced Spawning (Out-of theßSeason Spawning); Theoretical Background; State-of-the-Art ofß'Advanced Spawning' inßtheßEurasian Perch; Practical Recommendations; Broodstock Management; Photo-Thermal Manipulation; Stimulation ofßOvulation andßSpermiation; References; Appendices; Appendix 1.