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Preface; Contents; 1 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 1: The Basics; 1.1 Basic Concepts, with a Minimum of Annoying Physics: The Golden Rules; 1.1.1 Golden Rule 1: The Fluorescence Is Always at Longer Wavelengths than the Excitation; 1.1.2 Golden Rule 2: The Shape of the Emission Spectrum Does Not Change as the Excitation Wavelength Is Varied; 1.1.2.1 Quantum Yield; 1.1.2.2 Measuring Fluorescence Intensities and Spectra; 1.1.3 Golden Rule 3: Ordinarily, the Excitation Spectrum Is the Same Shape as the Absorption Spectrum; Glossary.
2 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 2: Using Fluorescence Anisotropy or Polarization to View Intermolecular Associations2.1 What's Behind Fluorescence Anisotropy?; 2.2 To Understand Fluorescence Anisotropy or Polarization, You Need to Know One Simple Equation; 2.3 To Measure Fluorescence Anisotropy or Polarization, You Need to Think About Light; References; 3 Time-Gated FRET Detection for Multiplexed Biosensing; 3.1 Introduction; 3.2 Theory of Time-Gated (TG) FRET; 3.2.1 Time-Gated vs. Time-Resolved Luminescence.
3.2.2 TG FRET (Förster Resonance Energy Transfer)3.2.3 Multiplexed TG FRET; 3.3 Advantages of TG FRET; 3.3.1 Single Analyte Detection; 3.3.2 Multiplexed Detection; 3.4 Spectroscopy-Based TG FRET Biosensing; 3.4.1 Equipment and Detection Formats; 3.4.2 Single Analyte Biosensing Applications; 3.4.3 Multiplexed Biosensing Applications; 3.5 Microscopy-Based TG FRET Biosensing; 3.5.1 Equipment and Detection Formats; 3.5.2 TG Luminescence Biosensing; 3.5.3 TG FRET Biosensing; 3.6 Conclusions and Future Perspectives; References.
4 Pharmacologically Active Plant Flavonols as Proton Transfer Based Multiparametric Fluorescence Probes Targeting Biomolecules: Perspectives and Prospects4.1 Introduction: Background and Historical Perspectives; 4.1.1 Fluorescence as an Exquisitely Sensitive Approach for Exploring Biomolecular Interactions: A Brief Overview; 4.1.2 Plant Flavonoids: Natural Distribution, Chemical Classification, Therapeutic Importance, and Intrinsic Fluorescence Behavior.
4.1.3 Excited-State Intramolecular Proton Transfer (ESIPT) and `Two Color' Fluorescence of Flavonols: Historical Perspective and Sensitivity to Environment4.2 Applications of ESIPT and `Two Color' Fluorescence of Therapeutically Active Flavonols with Representative Bio-relevant Targets: Scope of the Present Survey and Fluorescence Methodologies; 4.3 Protein-Flavonol Interactions; 4.3.1 Background; 4.3.2 Serum Albumin-Flavonol Interactions; 4.3.3 Hemoglobin-Flavonol Interaction; 4.4 DNA-Flavonol Interactions; 4.4.1 Background; 4.4.2 Duplex DNA-Flavonol Interactions.
2 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 2: Using Fluorescence Anisotropy or Polarization to View Intermolecular Associations2.1 What's Behind Fluorescence Anisotropy?; 2.2 To Understand Fluorescence Anisotropy or Polarization, You Need to Know One Simple Equation; 2.3 To Measure Fluorescence Anisotropy or Polarization, You Need to Think About Light; References; 3 Time-Gated FRET Detection for Multiplexed Biosensing; 3.1 Introduction; 3.2 Theory of Time-Gated (TG) FRET; 3.2.1 Time-Gated vs. Time-Resolved Luminescence.
3.2.2 TG FRET (Förster Resonance Energy Transfer)3.2.3 Multiplexed TG FRET; 3.3 Advantages of TG FRET; 3.3.1 Single Analyte Detection; 3.3.2 Multiplexed Detection; 3.4 Spectroscopy-Based TG FRET Biosensing; 3.4.1 Equipment and Detection Formats; 3.4.2 Single Analyte Biosensing Applications; 3.4.3 Multiplexed Biosensing Applications; 3.5 Microscopy-Based TG FRET Biosensing; 3.5.1 Equipment and Detection Formats; 3.5.2 TG Luminescence Biosensing; 3.5.3 TG FRET Biosensing; 3.6 Conclusions and Future Perspectives; References.
4 Pharmacologically Active Plant Flavonols as Proton Transfer Based Multiparametric Fluorescence Probes Targeting Biomolecules: Perspectives and Prospects4.1 Introduction: Background and Historical Perspectives; 4.1.1 Fluorescence as an Exquisitely Sensitive Approach for Exploring Biomolecular Interactions: A Brief Overview; 4.1.2 Plant Flavonoids: Natural Distribution, Chemical Classification, Therapeutic Importance, and Intrinsic Fluorescence Behavior.
4.1.3 Excited-State Intramolecular Proton Transfer (ESIPT) and `Two Color' Fluorescence of Flavonols: Historical Perspective and Sensitivity to Environment4.2 Applications of ESIPT and `Two Color' Fluorescence of Therapeutically Active Flavonols with Representative Bio-relevant Targets: Scope of the Present Survey and Fluorescence Methodologies; 4.3 Protein-Flavonol Interactions; 4.3.1 Background; 4.3.2 Serum Albumin-Flavonol Interactions; 4.3.3 Hemoglobin-Flavonol Interaction; 4.4 DNA-Flavonol Interactions; 4.4.1 Background; 4.4.2 Duplex DNA-Flavonol Interactions.